Although modern cherry sweet cherry production favours self-compatible varieties based on S-allele mutations, the majority of sweet cherry varieties still belong to the group of self-incompatible varieties. This is especially true for traditional local varieties and all of the old world-known varieties. Therefore, the full description of varieties includes knowledge about the composition of S-alleles, which is a prerequisite for setting up the production and the choice of suitable pollinators. The analysis of S-alleles was carried out for eight genotypes from the region of Banja Luka, alongside with three Slovenian varieties and several world known varieties which were included as referral genotypes. The DNA extracted from young leaf tissue was used for the detection of S-alleles by molecular markers. The detection of S-alleles was carried on by PCR amplification of hypervariable segments of S-loci. The fragments of DNA were separated by electrophoresis in the agarose gel and compared with the fragments of referral genotypes, with the standard DNA for the estimation of the length of the PCR amplified fragments, and with literature data. The composition of S-alleles was determined in all genotypes included into the research. A unique combination of S-alleles, S10S13, not known in the literature until now, was determined for one genotype (Ašlamka 2) from Bosnia. For all the genotypes, which are self-incompatible, a recommendation for the most suitable varieties-pollinators was made among the available varieties