| Abstract | Vinova loza bez sumnje predstavlja jednu od gospodarski najvažnijih kultura koja se uzgaja širom svijeta. Na profitabilnosti proizvodnje te kvalitetu uroda, između ostalih skupina patogena, utječu i virusi kojih je do danas kod loze opisano 86 iz 18 porodica i 35 rodova. U ovome radu testirani su metodom ELISA uzorci rozgve pet autohtonih hrvatskih sorata vinove loze iz pokušališta „Jazbina“ na osam gospodarski značajnih virusa: virus mozaika gušarke (ArMV), virus lepezastog lista vinove loze (GFLV), uvijenosti lista vinove loze pridruženi virus 1 (GLRaV-1), uvijenosti lista vinove loze pridruženi virus 2 (GLRaV-2), uvijenosti lista vinove loze pridruženi virus 3 (GLRaV-3), A-virus vinove loze, B-virus vinove loze te virus pjegavosti lista vinove loze (GFkV). Testiranjem je obuhvaćeno ukupno 472 uzorka slijedećih sorata: Debit (23 uzorka), Maraština (47 uzoraka), Plavina (79 uzoraka), Plavac mali (153 uzorka) te Pošip (170 uzoraka). Dokazana je prisutnost šest od osam virusa, odnosno ArMV, GFkV, GLRaV-1, GLRaV-2, GLRaV-3, GVA, dok prisutnost GFLV i GVB nije potvrđena. Najveći postotak kolekcijskog nasada bio je zaražen s GFkV (28%), slijedili su ga GVA (8%), zatim GLRaV-2 i GLRaV-3 (2%), dok je najmanja zaraza utvrđena sa ArMV (manje od 1 %). Najlošije zdrastveno stanje pokazala je sorta Debit gdje je od 23 trsova, broj biljaka slobodnih od virusa iznosio četiri (17%), dok je najbolje zdravstveno stanje u pogledu zaraze virusima utvrđeno kod sorte Plavina sa 79 (100%) trsova slobodnih od testiranih virusa. Od 472 testirana uzorka, na više od 30% nije identificiran niti jedan virus što ukazuje na kvalitetu sadnog materijala, dobru vinogradsku praksu i pridržavanje preventivnih mjera. |
| Abstract (english) | The vine is without a doubt one of the most economically important crops grown around the world. The profitability of production and the quality of the crop, among other groups of pathogens, are also influenced by viruses, of which 86 from 18 families and 35 genera have been described in the vine to date. In this work, ELISA samples of five autohtonus grapevine cultivars from the Jazbina experimental site were tested for eight economically significant viruses: arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV), grapevine leafroll-associated virus 1 (GLRaV-1), grapevine leafroll-associated virus 2 (GLRaV-2), l grapevine leafroll-associated virus 3 (GLRaV-3), grapevine virus A, grapevine virus B and grapevine fleck virus (GFkV). The testing included a total of 472 samples of the following varieties: Debit (23 samples), Maraština (47 samples), Plavina (79 samples), Plavac mali (153 samples), and Pošip (170 samples). The presence of six of eight viruses, ArMV, GFkV, GLRaV-1, GLRaV-2, GLRaV-3, GVA, was proven, while the presence of GFLV and GVB was not confirmed. The highest percentage of collection plantations was infected with GFkV (28%), followed by GVA (8%), GLRaV-2 and GLRaV-3 (2%), while the lowest infection was found with ArMV (less than 1%). The worst health condition was shown by the variety Debit, where out of 23 samples, the number of plants free of viruses was four (17%) while the best health condition was shown by variety Plavina with 100% of vines free of viruses. Of the 472 samples tested, no virus was identified in more than 30%, indicating the quality of planting material, good agricultural practice and adherence to preventive measures. |
