At present, cities are focal points of interaction between urbanization and nature. City open spaces are areas of some particular purpose, and plant material in such spaces may be present in a greater or lesser extent. Herbal material is primarily a living, growing and dynamic element, or constantly changing with regard to the season and growth. The ratio of alohton and autochthonous plant species is often insufficiently and disproportionately thought-out, reducing the environmental and other potentials of plant-based landscaping. If we follow the trends of the profession, there is an increasing interest in introducing indigenous species in the design of public and private spaces because they require less maintenance, and there is growing interest in introducing autochthonous species in the design of public and private spaces, and the growing importance of biodiversity in urban environments is increasingly represented. One of the objectives of the study was to establish the presence of autochthonous and alohton plant species in selected urban and rural locations and areas. By reviewing the ‘Katastar zelenila’, using literature and field visitation, it has been identified 197 plant species at 15 locations in the City of Zagreb, of which 12 locations are urban parks and 3 locations of rural features. Plant species are categorized into two groups; shrubs and trees. In both categories the average of autochthonous species is higher than the average of alohton species. The total number of autochthonous species identified is 54,70%, and the total number of alohton species is 45,30%. The final product of this paper is the Proposal of Indigenous Plant Material for Landscape Design, which describes 54 plant species and 28 different genera that are divided into 4 different categories, together with the description and image of each plant species.