Strawberry is a significant seasonal fruit, rich in nutrients and bioactive substances, which is why it is often called a super fruit. In terms of agro-technical complexity, it is very demanding, intensive crop and is one of the most sensitive fruit species in which fertilization has great significance, like all other agricultural cultures. A significant trace element in strawberry fertilization is iron. Strawberry is sensitive to its deficiency whereby sensitivity varies between varieties. Iron is essential for protein synthesis, sulfate and nitrate reduction, electron transport, it is constituent of cytochrome, catalase, ferredoxin, it is cofactor of approximately 140 enzymes, plays a significant role in basic biological processes such as photosynthesis and nitrogen fixation, and is integral part of all plant redox systems. Three different treatments, Fe/Zn coated with alginate, Fe/Zn coated with alginate and chitosan, and foliar iron application /Fe-EDDHA) were applied to Clery and Joly strawberries to determine the effect of this on yield and on the different properties of strawberry fruits. The experiment was set in 2019. in 4 repetitions per random block schedule in Botinec. The treatments did not significantly increase the amount of total soluble solids, and only slightly increased the yield and number of fruits. Three different treatments had an effect on total acidity, firmness and color of the fruit, with no uniform effect of each treatment on these properties depending on the harvest and strawberry variety. Fe/Zn coated with alginate is the only treatment that had a significant positive effect on the amount of bioactive substances and antioxidant activity of fruits in the Joly variety.