Pollen beetles (Brassicogethes spp.) are considered as one of the most important pests of oilseed rape. By feeding on plants, they damage closed flower buds. Successive sowing of oilseed rape on the same plots has led to an increase in the population of pollen beetles, and thus increased the use of insecticides to control pollen beetles. The emergence of resistance of pollen beetles to the active substances most commonly used in the control of pollen beetles is one of the most important problems in the cultivation of oilseed rape. Adult pollen beetles were collected during 2021 at the Šašinovec experimental site, Faculty of Agriculture University of Zagreb. Sensitivity tests of adult pollen beetles were performed in the laboratory according to IRAC test methods. The active substances of the insecticides used in the testing were lamba-cyhalothrin and acetamiprid as chemical insecticides, and azadiractin and pyrethrin as botanical insecticides. The test results showed that the pollen beetle population at the Šašinovec locality are sensitive to the active substance lambda-cyhalothrin, moderately resistant to the active substance acetamiprid, resistant to the active substance azadirachtin and highly sensitive to the active substance pyrethrin. Botanical insecticides have shown a good insecticidal effect. It would be desirable to continue research on the susceptibility of pollen beetle population to botanical insecticides, in order to confirm with greater certainty their potential application in pollen beetles’ control.