Genotipizacija varaždinskog kupusa pomoću AFLP markera

Maretić, Renato

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master's thesis

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Genotyping of 'Varaždinski' cabbage using AFLP markers

Renato Maretić (2016)

University of Zagreb
Faculty of Agriculture
Department of Plant Breeding Genetics, Biometrics and Experimentation

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APA 6th Edition

Maretić, R. (2016). Genotipizacija varaždinskog kupusa pomoću AFLP markera (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:868959

MLA 8th Edition

Maretić, Renato. "Genotipizacija varaždinskog kupusa pomoću AFLP markera." Master's thesis, University of Zagreb, Faculty of Agriculture, 2016. https://urn.nsk.hr/urn:nbn:hr:204:868959

Chicago 17th Edition

Maretić, Renato. "Genotipizacija varaždinskog kupusa pomoću AFLP markera." Master's thesis, University of Zagreb, Faculty of Agriculture, 2016. https://urn.nsk.hr/urn:nbn:hr:204:868959

Harvard

Maretić, R. (2016). 'Genotipizacija varaždinskog kupusa pomoću AFLP markera', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 22 April 2019, https://urn.nsk.hr/urn:nbn:hr:204:868959

Vancouver

Maretić R. Genotipizacija varaždinskog kupusa pomoću AFLP markera [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2016 [cited 2019 April 22] Available at: https://urn.nsk.hr/urn:nbn:hr:204:868959

IEEE

R. Maretić, "Genotipizacija varaždinskog kupusa pomoću AFLP markera", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:204:868959

Cite this item: https://urn.nsk.hr/urn:nbn:hr:204:868959

Metadata

Title	Genotipizacija varaždinskog kupusa pomoću AFLP markera
Title (english)	Genotyping of 'Varaždinski' cabbage using AFLP markers
Author	Renato Maretić
Mentor	Snježana Bolarić (mentor)
Committee member	Snježana Bolarić (predsjednik povjerenstva)
Committee member	Ivanka Žutić (član povjerenstva)
Committee member	Snježana Kereša (član povjerenstva)
Granter	University of Zagreb Faculty of Agriculture (Department of Plant Breeding Genetics, Biometrics and Experimentation) Zagreb
Defense date and country	2016-06-27, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Agronomy
Abstract	Tradicionalni kultivar 'Varaždinski kupus' od velikog je gospodarskog i kulturnog značaja. Njegovim sjemenarstvom bavi se još samo nekoliko obiteljskih poljoprivrednih gospodarstava, uglavnom za vlastite potrebe. Komercijalno dostupno sjeme je stranog porijekla i upitne srodnosti. Srodnost je proučavana pomoću AFLP molekularnih markera za čije proučavanje su bile korištene četiri polimorfne početnice. Proučavano je pet populacija: Varaždinski kupus iz Nacionalne banke biljnih gena (Var), populacije Varaždinskog kupusa obiteljskih gospodarstava OPG Hladika (Hld), OPG Plantić (Pln), OPG Cafuk (Caf) i komercijalno dostupno sjeme talijanske sjemenske kuće „Vita Bella“ (Vit). Detektirano je ukupno 203 markera od čega ih je 174 (86%) bilo polimorfnih. Analizom molekularne varijance utvrđena je signifikantna razlika između proučavanih populacija (ΦST:0.21282; p<0.001). Najveći udio fenotipske varijance opada na variranje unutar populacija (78.72%). Analizom međupopulacijskih udaljenosti utvrđena je signifikantna razlika između svih ispitivanih populacija (p<0,05%). Najveća udaljenost utvrđena je između populacija Vit i Pln (0,23), a najmanja udaljenost utvrđena je između populacija Caf i Pln (0,09). Analizom glavnih koordinata utvrđeno je grupiranje individua proučavanih populacija Varaždinskog kupusa u dvije glavne skupine. Jednu skupinu čine individue populacija Vit, a drugu populacije Var, Hld, Pln i Caf. Rezultati istražvanja važan su doprinos u poznavanju genetske varijabilnosti populacija Varaždinskog kupusa koji se mogu iskoristiti za zasnivanje organiziranog sjemenarstva Varaždinskog kupusa u Republici Hrvatskoj.
Abstract (english)	Traditional landrace of ‘Varaţdinski’ cabbage is of a great cultural and economical meaning. There are only few family farms that are producing its seed, mainly for personal use. Commercially available seed is produced in foreign countries and has questionable origin. Genetic similarity is analyzed by AFLP molecular markers with four polymorphic primers. Five populations have been studied: ‘Varaždinski’ cabbage from National bank of plant genes (Var), populations of ‘Varaždinski’ cabbage from family farms OPG Hladika (Hld), OPG Plantić (Pln), OPG Cafuk (Caf) and commercially available seed from Italian seed production company ‘Vita Bella’. It has been detected in total of 203 markers and 174 (86%) were polymorphic. By analysis of molecular variance we have established significant difference between studied populations (ΦST:0.21282; p<0.001). Most of the phenotypic variance belongs to variance between populations (78.72%). Analysis of distance between populations determined significant differences among analyzed populations (p<0,05%). Biggest distance was found between populations Vit and Pln (0,23), and the smallest was between populations Caf and Pln (0,09). Principal Coordinate Analysis defined clear vertical separation of two main groups. Population Vit forms first group and populations Var, Hld, Pln and Caf form the other group. The results of this research make a great contribution for gene variability of ‘Varaždinski’ cabbage and can be used for creating commercial seed production of ‘Varaždinski’ cabbage in Republic of Croatia.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:204:868959
Study programme	Title: Biljne znanosti Study programme type: sveučilišni Study level: diplomski Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
Type of resource	text
File origin	Born digital
Access conditions	Restricted access
Terms of use
Repository	Repository Faculty of Agriculture University of Zagreb
Created on	2016-08-29 12:13:36