Genetska struktura tradicionalnih sorata glavatog kupusa (Brassica oleracea L. var. capitata f. alba)

Ćurak, Josip

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master's thesis

Genetska struktura tradicionalnih sorata glavatog kupusa (Brassica oleracea L. var. capitata f. alba)

Zagreb: University of Zagreb, Faculty of Agriculture, 2021. urn:nbn:hr:204:611556

Ćurak, Josip

University of Zagreb
Faculty of Agriculture
Department of Plant Breeding Genetics, Biometrics and Experimentation

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APA 6th Edition

Ćurak, J. (2021). Genetska struktura tradicionalnih sorata glavatog kupusa (Brassica oleracea L. var. capitata f. alba) (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:611556

MLA 8th Edition

Ćurak, Josip. "Genetska struktura tradicionalnih sorata glavatog kupusa (Brassica oleracea L. var. capitata f. alba)." Master's thesis, University of Zagreb, Faculty of Agriculture, 2021. https://urn.nsk.hr/urn:nbn:hr:204:611556

Chicago 17th Edition

Harvard

Ćurak, J. (2021). 'Genetska struktura tradicionalnih sorata glavatog kupusa (Brassica oleracea L. var. capitata f. alba)', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 31 March 2023, https://urn.nsk.hr/urn:nbn:hr:204:611556

Vancouver

Ćurak J. Genetska struktura tradicionalnih sorata glavatog kupusa (Brassica oleracea L. var. capitata f. alba) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2021 [cited 2023 March 31] Available at: https://urn.nsk.hr/urn:nbn:hr:204:611556

IEEE

J. Ćurak, "Genetska struktura tradicionalnih sorata glavatog kupusa (Brassica oleracea L. var. capitata f. alba)", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:204:611556

Cite this item: https://urn.nsk.hr/urn:nbn:hr:204:611556

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Metadata

Title	Genetska struktura tradicionalnih sorata glavatog kupusa (Brassica oleracea L. var. capitata f. alba)
Title (english)	Genetic structure of traditional varieties of head cabbage (Brassica oleracea L. var. capitata f. alba)
Author	Josip Ćurak
Mentor	Snježana Bolarić (mentor)
Committee member	Snježana Bolarić (predsjednik povjerenstva)
Committee member	Aleš Vokurka (član povjerenstva)
Committee member	Ivanka Žutić (član povjerenstva)
Granter	University of Zagreb Faculty of Agriculture (Department of Plant Breeding Genetics, Biometrics and Experimentation) Zagreb
Defense date and country	2021-09-23, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Agronomy
Abstract	Tradicionalne sorte glavatog kupusa od velikog su gospodarskog i kulturnog značaja. Analizirane su četiri udomaćene (´Braunschweiger´, ´Kopenhaški´, ´Ditmar´, ´Slava´) i pet domaćih sorata (´Čepinski´, ´Varaždinski´, ´Ogulinski´, ´Žminjski´, ´Brgujski´). Varijabilnost germplazme je proučavana pomoću AFLP molekularnih markera da bi mogli utvrditi varijabilnost unutar sorata i između 9 sorata. Utvrđivanje varijabilnosti je cilj ovog istraživanja. Kako bi utvrdili varijabilnost koristili smo molekularne rezultate za analizirane sorte, te usporedili i napravili procjenu varijabilnosti na molekularnoj razini. Sjetva sjemena je obavljena početkom listopada 2019., a sjeme je sijano u kontejnere i čuvano u kontroliranim uvjetima. Od 9 proučavanih sorata analizirano je unutar svake sorte po 20 individua, a sveukupno 180 individua (9 sorti x 20 individua). U AFLP analizi su korištene četiri kombinacije početnica. Primarno je bilo potrebno napraviti izolaciju DNA, a kasnije je to pomoglo u analizi genetske strukture za koju su korišteni AFLP markeri. Matrica različitosti korištena je u analizi molekularne varijance (AMOVA), analizi udaljenosti između sorata i u STRUKTURE analizi.
Abstract (english)	Traditional varieties of head cabbage are of great economic and cultural importance. Four domesticated varieties (´Braunschweiger´, ´Copenhaški´, ´Ditmar´, ´Slava´) and five domestic varieties (´Čepinski´, ´Varaždinski´, ´Ogulinski´, ´Žminjski´, ´Brgujski´) were analyzed. Germplasm variability was studied using AFLP molecular markers to be able to determine variability within cultivars and between 9 cultivars. Determining variability is the goal of this research. To determine variability, we used molecular results for the analyzed cultivars, and compared and estimated variability at the molecular level. Seeds were sown in early October 2019, and the seeds were sown in containers and stored under controlled conditions. Of the 9 cultivars studied, 20 individuals were analyzed within each cultivar, and a total of 180 individuals (9 cultivars x 20 individuals). AFLP analysis was done using four AFLP primer combinations. Primary DNA isolation was required, and later this helped in the analysis of the genetic structure for which AFLP markers were used. The diversity matrix was used in molecular variance analysis (AMOVA), cultivar distance analysis, and STRUCTURE analysis.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:204:611556
Study programme	Title: Plant Sciences Study programme type: university Study level: graduate Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository Faculty of Agriculture University of Zagreb
Created on	2021-11-22 08:51:14