Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus

Malović, Helena

prikaz prve stranice dokumenta Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus

Access restricted to students and staff of home institution

master's thesis

Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus

Zagreb: University of Zagreb, Faculty of Agriculture, 2021. urn:nbn:hr:204:775425

Malović, Helena

University of Zagreb
Faculty of Agriculture
Department of Plant Pathology

Cite this document

APA 6th Edition

Malović, H. (2021). Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:775425

MLA 8th Edition

Malović, Helena. "Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus." Master's thesis, University of Zagreb, Faculty of Agriculture, 2021. https://urn.nsk.hr/urn:nbn:hr:204:775425

Chicago 17th Edition

Harvard

Malović, H. (2021). 'Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 28 March 2023, https://urn.nsk.hr/urn:nbn:hr:204:775425

Vancouver

Malović H. Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2021 [cited 2023 March 28] Available at: https://urn.nsk.hr/urn:nbn:hr:204:775425

IEEE

H. Malović, "Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:204:775425

Cite this item: https://urn.nsk.hr/urn:nbn:hr:204:775425

Please login to the repository to save this object to your list.

Metadata

Title	Simptomatologija i karakterizacija izolata fitopatogene bakterije Pseudomonas syringae iz tkiva biljaka roda Prunus
Title (english)	Simptomatology and characterization of isolates of phytopathogenic bacteria pseudomonas syringae from plants of genus Prunus tissues
Author	Helena Malović
Mentor	Edyta Đermić (mentor)
Committee member	Edyta Đermić (predsjednik povjerenstva)
Committee member	Joško Kaliterna (član povjerenstva)
Committee member	Martina Skendrović Babojelić (član povjerenstva)
Granter	University of Zagreb Faculty of Agriculture (Department of Plant Pathology) Zagreb
Defense date and country	2021-09-30, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Agronomy
Abstract	Bakterija Pseudomonas syringae je uzročnik raka koštičavih voćnih vrsta koji se smatra jednom od najvažnijih bolesti koštičavih voćnih vrsta. Karakteristični, golim okom vidljivi simptomi zaraze su pojava rana na granama ili deblu uz obilno lučenje smole, a kod starijih stabala kora može biti ispucala, udubljena ili promijenjene boje. Problem predstavljaju latentne zaraze bakterijom P. syringae kada nema uočljivih simptoma, te se javlja potreba za izolacijom bakterije iz tkiva biljaka nakon čega slijedi njena identifikacija različitim testovima koji potvrđuju da se radi o bakteriji P. syringae i jasni su pokazatelj zaraze. U radu je dan prikaz simptoma bolesti raka koštičavih voćnih vrsta, te postupak i metode testiranja karakteristika izoliranih bakterijskih izolata iz prikupljenih uzoraka tkiva biljaka iz roda Prunus, s područja grada Zagreba. Posebno su istaknuti provedeni testovi hipersenzitivne reakcije na duhanu i biotest na jorgovanu. Rezultati testova su pokazali da je bakterija P. syringae uspješno izolirana u slučaju svih 8 klonskih linija bakterija čime je potvrđena hipoteza da izolati iz uzoraka s jače izraženim simptomima pokazuju izrazitu patogenost. Rad donosi i smjernice za nastavak determinacije bakterijskih izolata u smjeru određivanja zasebnih patovara i sojeva.
Abstract (english)	Bacterium Pseudomonas syringae is the causing agent of bacterial cancer of stone fruits, which is one of the most importante diseases of stone fruits. Characteristical symptoms of infection, visible by naked eye, are appearing in form of wounds on branches or tree trunk accompanied with huge production of gummosis, whether the bark of the older trees can be cracked, concaved or, of changed colour. The problem are latent infections with bacteria P. syringae when there are no visible symptoms, so there is a need for isolation of bacteria from plant tissues and tests that confirm bacterium P. syringae and are clear sign of the infection. In this work symptoms of bacterial cancer of stone fruit disease are described, as well as procedures and testing methods of characteristic properties of bacteria isolated from gathered tissue samples of plants of genus Prunus, from the Zagreb city area. Implementation of hypersensitive reaction on tobacco and biotest on lilac werw emphasized. Tests results showed that the bacteria P. syringae was successfully isolated in all of 8 clone bacterial lines which confirmed hypothesis that isolates from samples with stronger symptoms are showing stronger patogenicity. The thesis is giving directions for continuing of determination of bacterial isolates to separate pathovars and strains.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:204:775425
Study programme	Title: Phytomedicine Study programme type: university Study level: graduate Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository Faculty of Agriculture University of Zagreb
Created on	2021-12-03 12:49:39