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master's thesis
Detekcija i djelomična molekularna karakterizacija A-virusa vinove loze iz zeljastih domaćina
Zagreb: University of Zagreb, Faculty of Agriculture, 2021. urn:nbn:hr:204:367360
Mumelaš, Monika
University of Zagreb
Faculty of Agriculture
Department of Plant Pathology

Cite this document

Mumelaš, M. (2021). Detekcija i djelomična molekularna karakterizacija A-virusa vinove loze iz zeljastih domaćina (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:367360

Mumelaš, Monika. "Detekcija i djelomična molekularna karakterizacija A-virusa vinove loze iz zeljastih domaćina." Master's thesis, University of Zagreb, Faculty of Agriculture, 2021. https://urn.nsk.hr/urn:nbn:hr:204:367360

Mumelaš, Monika. "Detekcija i djelomična molekularna karakterizacija A-virusa vinove loze iz zeljastih domaćina." Master's thesis, University of Zagreb, Faculty of Agriculture, 2021. https://urn.nsk.hr/urn:nbn:hr:204:367360

Mumelaš, M. (2021). 'Detekcija i djelomična molekularna karakterizacija A-virusa vinove loze iz zeljastih domaćina', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:204:367360

Mumelaš M. Detekcija i djelomična molekularna karakterizacija A-virusa vinove loze iz zeljastih domaćina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2021 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:204:367360

M. Mumelaš, "Detekcija i djelomična molekularna karakterizacija A-virusa vinove loze iz zeljastih domaćina", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:204:367360

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Metadata
TitleDetekcija i djelomična molekularna karakterizacija A-virusa vinove loze iz zeljastih domaćina
Title (english)Detection and partial molecular characterization of grapevine virus A form herbaceous hosts
AuthorMonika Mumelaš
MentorDarko Vončina (mentor)
Committee memberDarko Vončina (predsjednik povjerenstva)
Committee memberEdyta Đermić (član povjerenstva)
Committee memberIvica Ljubičić (član povjerenstva)
Committee memberMartin Jagunić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Department of Plant Pathology)
Zagreb
Defense date and country2021-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Vinova loza je domaćin velikog broja virusa od kojih je jedan od ekonomski najznačajnijih A-virus vinove loze (Grapevine virus A, GVA), a osim sadnim materijalom može se širiti štitastim ušima na vinovu lozu i na zeljaste domaćine. Za potrebe ovog rada istraženi su prijenos i prisutnost GVA u zeljastim biljkama metodama ELISA, konvencionalnim RT-PCR-om i PCR-om u realnom vremenu. Pozitivne zeljaste biljke na GVA bile su oštrodlakavi šćir (Amaranthus retroflexus), Nicotiana benthamiana, loboda kamenjarka (Chenopodium murale) i mak turčinjak (Papaver rhoeas). Odabrano je 9 GVA izolata koji su sekvencirani, a dobivene sekvence uspoređene su međusobno te s drugim izolatima iz GenBanke. Dobivene sekvence razlikuju se u nukleotidnom slijedu gdje se sastoje od 327 monomorfnih i 63 polimorfnih mjesta, dok na aminokiselinskoj razini nije došlo do značajnih promjena. Sekvence izvorišne biljke dokazale su prisutnost više GVA varijanata. Na filogenetskom stablu, istraživani GVA izolati grupirali su se u dvije zasebne skupine.
Abstract (english)
The grapevine can be attacked by a large number of viruses of which the most important is Grapevine virus A (GVA) which can be spread with both planting materials and by mealybugs on grapes and herbaceous hosts. For the purposes of this research, the transmission and presence of GVA in herbaceous plants were investigated by ELISA, conventional RT-PCR and Real-Time PCR methods. Plants that tested positive for GVA were redroot pigweed (Amaranthus retroflexus), Nicotiana benthamiana, nettle-leaved goosefoot (Chenopodium murale) and common poppy (Papaver rhoeas). Nine isolates were selected and sent for sequencing. The obtained sequences were compared with each other and with other isolates from GenBank. The resulting sequences differ in the nucleotide sequence; they consist of 327 monomorphic and 63 polymorphic sites, while at the amino acid level there were no significant changes. Source plant sequences proved the presence of multiple GVA variants. Within the phylogenetic tree tested GVA isolates were gathered in two distinct groups.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:367360
Study programmeTitle: Phytomedicine
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository Faculty of Agriculture University of Zagreb
Created on2021-12-09 10:52:49

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