Aluminum (Al) is the third most abundant element in the Earth's crust, and its dissolution increases at acidic pH. Acidic soils occupy a large part of the world's arable soils, and aluminum toxicity occurs at a pHH2O lower than 5,5. Growing corn (Zea mays L.) on acidic soil can cause yield reductions of more than 50%. The toxicity of Al3+ is manifested by the inhibition of the growth of the root system, but the symptoms can also be observed on the above-ground organs. The aim of the work was to determine the physiological changes in the above-ground organs of corn grown under conditions of toxic concentrations of aluminum using nondestructive methods of multispectral analysis. The research was conducted in controlled conditions of growth chambers, on a total of 50 plants grown in nutrient solutions with different concentrations of aluminum (0, 50, 100, 200 and 400 µM dm-3 AlCl3). Multispectral imaging of each plant was performed after 13 days of cultivation in the treatments, using the CropReporter® device (PhenoVation, Wageningen, the Netherlands). Treatments with aluminum in concentrations of 100 µM dm-3, 200 µM dm-3 and 400 µM dm-3, caused reduction of vegetation indices is visible, which indicates direct or indirect toxic effects of Al on the photosynthetic pigments. With the increase in aluminum concentration, the reflection in the RRed, RGreen, RBlue, RSpcGrn and RFarRed spectra also increased, which indicates that in conditions of aluminum toxicity there is a decrease in the absorption of light energy, which will consequently lead to a decrease in photosynthetic activity. This work show the usefulness multispectral analysis for the purpose of quantifying the phenotypic changes of above-ground organs of corn under conditions of aluminum toxicity.