The Greater Wax Moth (Galleria mellonella) is one of the most important pests of bee colonies, especially on combs stored outside the beekeeping season. It damages the comb and partly the undrawn comb foundation, bee pollen, and wooden parts of the hive. They also transmit various pathogens. Wax moth attacks are most common in early autumn and late spring. The aim of this study was to determine differences between populations of wax moths collected from conventional and organic production in Croatia using wing shape analysis. Geometric morphometrics methods were used for this purpose. Principal component analyses (PCA) and canonical discriminant analysis (CVA) were conducted. A total of 135 wing samples were analyzed, approximately with an equal ratio of males to females (48 : 52%). Specific markers were placed on the wax moth wings; 12 on the lower and 11 on the upper wings. No variability was found between sexes, that is, sexual dimorphism, indicating stable populations. Furthermore, differences in agroecological conditions and types of production do not significantly affect population variability, suggesting the presence of phenotypic plasticity and the ability to adapt to new habitats with stable genotypes. Variability in the size of the upper wings was observed, especially in females from the Zadar area. This finding suggests potential differences between inland and coastal populations, which can give this species a competitive advantage when spreading to new areas. Further research using geometric morphometrics on the G. mellonella species could better elucidate the connection between wing morphology and adaptability, thus providing a better foundation for the development of control strategies and preventing the spread to new environments.