The aim of the study is to determine the influence of different laser treatments on the activation of the germination potential of Dalmatian pyrethrum seeds. The average value of the germination energy of the control sample was 2.00 %. After the treatments, an increase in germination energy was observed in all samples treated with a 100 mW and a 500 mW laser. In the samples treated with a 200 mW laser, an increase in germination energy was observed in the samples treated for 60 and 120 s, while no change in germination energy was observed in the samples treated for 30 s compared to the control sample. The average value of standard germination for the control sample was 6.00 %. As with the germination energy, an increase in germination was observed in almost all treated samples, except for the samples treated for 60 s with a 500 mW laser, which showed a decrease in standard germination of 0.33 %. Considering the output power of the laser, it is observed that the treatment of 120 s with 100 and 500 mW lasers caused the highest increase in germination energy of 2.00 %, while the highest increase in standard germination of 4.00 % was recorded when treated with a 100 mW laser for a duration of 120 s. The research results show that the radiation intensity per seed is relatively low, indicating that the effect of the laser on the plant material is primarily through biostimulation.