Wild edible saprotrophic mushrooms play a significant role in the global diet due to their unique nutritional value and medicinal properties. These fungi break down organic substances and contribute to the circulation of nutrients in the ecosystem. In recent times, intensive urbanization and industrialization have contributed to elevated levels of heavy metals in soil systems that subsequently accumulate in saprotrophic fungi. The aim of the work was to determine chromium concentrations in the soil and bioaccumulation potential in five edible mushroom species: Agaricus campestris L., Armillaria mellea (Vahl) P.Kumm., Clitocybe inversa (Sowerby) Vizzini, Clitocybe nebularis (Batsch), P.Kumm., and Macrolepiota procera (Scop.) Singer. at seven locations in continental and coastal Croatia. Chromium concentration analysis was performed using inductively coupled plasma optical emission spectrometry (ICP-OES). The results showed that Cr was present in all soil samples in the range from 7.55 to 30.14 mg/kg-1, while the concentration in mushrooms was significantly lower in the range from 0.01 to 1.91 mg/kg-1, while for which the determined concentrations were significantly higher in the cap compared to the stem. Mushroom and soil samples from central Croatia had higher chromium levels than those from coastal Croatia. Despite the concentrations of chromium in some mushroom species >1, the values of the bioaccumulation factor and health risk index, including the dietary intake of chromium metal and the health risk index, were below the limit values. Therefore, it has been shown that there are no significant health risks when consuming mushrooms, and it emphasizes the importance of determining the chromium bioaccumulation potential of wild edible mushrooms, as well as possible health hazards associated with their consumption.