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master's thesis
Mikropropagacija kupine sorte "Reuben"
Zagreb: University of Zagreb, Faculty of Agriculture, 2017. urn:nbn:hr:204:867678
Zec, Martina
University of Zagreb
Faculty of Agriculture
Department of Plant Breeding Genetics, Biometrics and Experimentation

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Zec, M. (2017). Mikropropagacija kupine sorte "Reuben" (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:867678

Zec, Martina. "Mikropropagacija kupine sorte "Reuben"." Master's thesis, University of Zagreb, Faculty of Agriculture, 2017. https://urn.nsk.hr/urn:nbn:hr:204:867678

Zec, Martina. "Mikropropagacija kupine sorte "Reuben"." Master's thesis, University of Zagreb, Faculty of Agriculture, 2017. https://urn.nsk.hr/urn:nbn:hr:204:867678

Zec, M. (2017). 'Mikropropagacija kupine sorte "Reuben"', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 20 April 2024, https://urn.nsk.hr/urn:nbn:hr:204:867678

Zec M. Mikropropagacija kupine sorte "Reuben" [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2017 [cited 2024 April 20] Available at: https://urn.nsk.hr/urn:nbn:hr:204:867678

M. Zec, "Mikropropagacija kupine sorte "Reuben"", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:204:867678

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Metadata
TitleMikropropagacija kupine sorte "Reuben"
Title (english)Micropropagation of blackberry cultivar "Reuben"
AuthorMartina Zec
MentorSnježana Kereša (mentor)
Committee memberSnježana Kereša (predsjednik povjerenstva)
Committee memberMarija Pecina (član povjerenstva)
Committee memberDubravka Dujmović Purgar (član povjerenstva)
Committee memberAnita Bošnjak Mihovilović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Department of Plant Breeding Genetics, Biometrics and Experimentation)
Zagreb
Defense date and country2017-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Cilj ovog istraživanja bio je utvrđivanje protokola za uspješnu mikropropagaciju kupine sorte “Reuben”. Vegetacijski vršci izolirani su aseptično pod stereomikroskopom iz aksilarnih pupova i postavljeni na medij za uspostavljanje kulture (EM). Ispitivan je utjecaj različitih šećera u PM2 mediju u dvije varijante 30 g/L saharoze i 20 g/L glukoze + 10 g/L saharoze. Kako bi se utvrdila optimalna koncentracija regultora rasta za mikropropagaciju, istraživane su 4 varijante: 6-benzilaminopurin (BAP) u koncentracijama 0,3 mg/L i 1 mg/L te meta-Topolin (mT) (u jednakim koncentracijama). Zakorjenjivanje in vitro provedeno je u dvije varijante, na IAA mediju za zakorjenjivanje i na mediju bez hormona. Najveći broj izdanaka po eksplantatu (2,6) dobiven je na mediju s koncentracijom 1 mg/L BAP-a, a najveća duljina najdužeg izdanka (8,9 mm) na mediju s koncentracijom 0,3 BAP-a. Postotak zakorjenjivanja je bio veći (61%) na mediju za zakorjenjivanje bez hormona, ali postotak aklimatizacije je bio sličan u obadvije varijante (92% i 95%).
Abstract (english)
The aim of the present study was to establish the protocol for micropropagation of blackberry cultivar “Reuben”. In aseptic conditions using stereomicroscope, shoot tips were isolated from axillary buds and placed on medium (EM) to establish aseptic culture. The effect of different sugars on PM2 medium in the two variants of 30 g / L sucrose and 20 g / L glucose + 10 g / L sucrose was tested. In order to establish optimal variant of growth regulators 4 variants were experimented, 6- benzylaminopurine (BAP) in concentration of 0.3 mg/L and 1 mg/L and meta-Topolin (mT) in same concentrations. In vitro rooting was carried out in two experimental variants, on IAA rooting medium and on hormone free rooting medium. The greatest number of shoots with average of 2.6 were produced in medium containing 1 mg/L BAP and the maximum shoot length of 8.9 was produced in medium containing 0.3 mg/L BAP. The rooting percentage was higher (61%) on hormone free rooting medium but the acclimatization percentages for both variants were very similar (92% and 95%).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:867678
Study programmeTitle: Plant Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository Faculty of Agriculture University of Zagreb
Created on2017-10-23 12:35:44

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