BIOLOŠKI UČINCI IZOLATA DOBIVENIH KEMIJSKOM EKSTRAKCIJOM IZ CVIJETOVA KAMILICE (Camomila mattricaria)

Pervan, Martina

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master's thesis

BIOLOŠKI UČINCI IZOLATA DOBIVENIH KEMIJSKOM EKSTRAKCIJOM IZ CVIJETOVA KAMILICE (Camomila mattricaria)

Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2013. urn:nbn:hr:181:994395

Pervan, Martina

Josip Juraj Strossmayer University of Osijek
Department of biology

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APA 6th Edition

Pervan, M. (2013). BIOLOŠKI UČINCI IZOLATA DOBIVENIH KEMIJSKOM EKSTRAKCIJOM IZ CVIJETOVA KAMILICE (Camomila mattricaria) (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:994395

MLA 8th Edition

Pervan, Martina. "BIOLOŠKI UČINCI IZOLATA DOBIVENIH KEMIJSKOM EKSTRAKCIJOM IZ CVIJETOVA KAMILICE (Camomila mattricaria)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2013. https://urn.nsk.hr/urn:nbn:hr:181:994395

Chicago 17th Edition

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Pervan, M. (2013). 'BIOLOŠKI UČINCI IZOLATA DOBIVENIH KEMIJSKOM EKSTRAKCIJOM IZ CVIJETOVA KAMILICE (Camomila mattricaria)', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 08 December 2023, https://urn.nsk.hr/urn:nbn:hr:181:994395

Vancouver

Pervan M. BIOLOŠKI UČINCI IZOLATA DOBIVENIH KEMIJSKOM EKSTRAKCIJOM IZ CVIJETOVA KAMILICE (Camomila mattricaria) [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2013 [cited 2023 December 08] Available at: https://urn.nsk.hr/urn:nbn:hr:181:994395

IEEE

M. Pervan, "BIOLOŠKI UČINCI IZOLATA DOBIVENIH KEMIJSKOM EKSTRAKCIJOM IZ CVIJETOVA KAMILICE (Camomila mattricaria)", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:181:994395

Cite this item: https://urn.nsk.hr/urn:nbn:hr:181:994395

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Title	BIOLOŠKI UČINCI IZOLATA DOBIVENIH KEMIJSKOM EKSTRAKCIJOM IZ CVIJETOVA KAMILICE (Camomila mattricaria)
Title (english)	EFFCTS OF BIOLOGICAL ISOLATES OBTAINED BY CHEMICAL EXTRACTION OF CHAMOMILE FLOWERS (Camomile mattricaria)
Author	Martina Pervan
Mentor	Tatjana Belovari (mentor) MBZ: 209063
Committee member	Tatjana Belovari (predsjednik povjerenstva) MBZ: 209063
Committee member	Ljubica Glavaš-Obrovac (član povjerenstva) MBZ: 162671
Committee member	Ivna Štolfa Čamagajevac (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Department of biology Osijek
Defense date and country	2013-12-12, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Biološki uĉinak 12 ekstrakata iz cvjetova kamilice istražen je in vitro na staniĉnim linijama HuT-78, K562, HeLa, NCI-H358 i MDCK-I. Antiproliferativne sposobnosti ekstrakata i apigenina nakon 72 sata izlaganja stanica određene su MTT testom. Morfološke promjene analizirane su svjetlosnom mikroskopijom, a apoptoza je određena Annexin-V-FITC testom. Rast stanica znaĉajno je inhibiralo 3 ekstrakta u koncentracijama 0,2 i 0,5 mg/mL rezultirajući s više od 80 % inhibicije rasta HuT-78 i K562 stanica. Najmanje osjetljiva staniĉna linija na djelovanje ekstrakata je NCI-H358 kod koje je uoĉena inhibicija rasta od 60-65 %. Najizražajnije morfološke i apoptotiĉke promjene na HeLa stanicama izaziva ekstrakt 11 (IC50 = 0,098 mg/ml). Ispitivani izolat apigenina u odnosu na komercijalno dostupan apigenin pokazuje razliĉit obrazac antiproliferativnog djelovanja u odnosu na komercijalno dostupni uzorak što upućuje da apigenin nije jedina biološki aktivna tvar u ekstraktima. U zakljuĉku, ispitivani ekstrakti znaĉajno inhibiraju rast stanica i imaju sposobnost indukcije apoptoze. Nije uoĉena selektivnost u djelovanju izmeĊu tumorskih i normalnih stanica.
Abstract (english)	The biological effect of 12 extracts from chamomile flowers was investigated in vitro on cell lines HuT-78, K562, HeLa, NCI-H358 and MDCK-I. Antiproliferative abilities of extracts and apigenin after 72 hours of cell exposure were examined by MTT assay. Morphological changes were analyzed by light microscopy, and apoptosis was determined by Annexin V-FITC assay. Cell growth was significantly inhibited by 3 extracts at concentrations 0.2 and 0.5 mg/mL resulting in a more than 80 % growth inhibition HuT-78 and K562 cells. The least sensitive cell line on the effect of extracts is NCI - H358 in which is observed inhibition of growth of 60-65 %. Pronounced morphological and apoptotic changes in HeLa cells were caused by extract 11 (IC 50 = 0.098 mg/mL). Isolate of apigenin compared to commercially available apigenin shows different patterns of antiproliferative activity compared to a commercially available sample, indicating that apigenin is not the only biologically active substance in the extract. In conclusion, the tested extracts significantly inhibited the growth of cells and have the ability to induce apoptosis. There was no selectivity toward normal and tumor cells.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:181:994395
Study programme	Title: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology Course: Master of Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Department of biology, Josip Juraj Strossmayer University of Osijek
Created on	2017-01-30 08:04:14

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