By examining the spectrum of life forms of grasslands in eastern Croatia, the dominance of hemikryptophytes, which are typical for temperate climate, is visible. The contribution of terophytes, geophytes, chamaephytes is in accordance with a spectrum of life forms in areas of Slavonia and Baranja. Increased portion of phanerophytes in some localities indicate the beginning of the succession overgrowth of grasslands. By increasing humidity on grasslands, increases representation of hemikryptophytes in the spectrum of life forms, and decreases the sequence toward the drier grasslands. Inverted sequence is presented by therophyta, which is higher represented in the spectrum of life forms of dry grasslands. Minimum of biodeversity is presented by humid meadow (C.2.1, C.2.2.) and associations Rumici-Alopecuretum geniculati and Junco-Menthetum longifoliae, humid, nitrophilous grasslands and pasture. A lower number of species in the panonian steppic grasslands is mostly the result of very small fragments of associations found in eastern Croatia. Mesophilic meadows of Central Europe (C.2.3.) and humid and nitrophilous pastures represent associations with a large number of species and high biodiversity. In order to obtain an overall picture of the condition and problems in grasslands, by researching is necessary to include all three fragments and floral composition and variety of life forms and biodiversity in grassland associations.