undergraduate thesis
The effect of cadmium on antioxidant enzyme gene expression

Mira Dombi (2016)
Sveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
Metadata
TitleUčinak kadmija na ekspresiju gena antioksidativnih enzima
AuthorMira Dombi
Mentor(s)Ivna Štolfa Čamagajevac (thesis advisor)
Abstract
Akumulacija kadmija u biljkama uzrokuje različite fiziološke, biokemijske i strukturne promjene, dok selen koji se često koristi u postupku biofortifikacije biljaka, može znatno promijeniti odgovor biljke na tretman kadmijem. Glavi cilj ovog istraživanja bio je utvrditi utjecaj kadmija na ekspresiju gena antioksidativnih enzima u korijenu i izdancima klijanaca dvije sorte pšenice (Divana i Srpanjka) obogaćene selenom. Određivanje utjecaj kadmija na gene koji kodiraju glutation S-transferazu, superoksid-dismutazu i katalazu. Učinak kadmija na ekspresiju gena antioksidativnih enzima ovisio je o sorti pšenice, te o vrsti biljnog organa (korijen i izdanak).Tretman kadmijem najviše je utjecao na smanjenje ekspresije gena za glutation S-transferazu. Učinak kadmija na smanjenje ekspresije gena bio je intenzivniji u korijenu nego u izdancima. Sorta Divana pokazala se osjetljivijom na tretman kadmijem od sorte Srpanjka.
Keywordscadmium selenium Triticum aestivum antioxidative enzymes
Parallel title (English)The effect of cadmium on antioxidant enzyme gene expression
Committee MembersIvna Štolfa Čamagajevac (committee chairperson)
GranterSveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineNATURAL SCIENCES
Biology
Study programme typeuniversity
Study levelundergraduate
Study programmeUndergraduate University Study Programme in Biology (Bachelor of Science)
Academic title abbreviationuniv.bacc.biol.
Genreundergraduate thesis
Language Croatian
Defense date2016-09-20
Parallel abstract (English)
The accumulation of cadmium in plants cause a variety of physiological, biochemical and structural changes, while selenium that is often used in the process of biofortification of plants can significantly change the plants response to cadmium treatment. The main aim of this study was to determine the effect of cadmium on antioxidant enzyme gene expression in the roots and shoots of two varieties of wheat (Divana and Srpanjka) seedlings enriched with selenium. Enzymes that were observed in this study were glutathione S-transferase, Cu, Zn-Superoxid-Dismutase and catalase The effect of cadmium on antioxidant enzyme gene expression depended on the type of cultivar and the type of plant organ (root or shoot). Cadmium treatment affected the expression of glutathione S-transferase the most.The effect of cadmium on decrease in antioxidant enzyme gene expression was much more visible in the roots than in the shoots. Variety Divana was shown to be more sensitive to cadmium treatment than variety Srpanjka.
Parallel keywords (Croatian)kadmij selen Triticum aestivum antioksidativni enzimi
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:181:519184
CommitterVesna Radman Meic