No public access
master's thesis
DIRECT POTENTIOMETRIC DETERMINATION OF DIASTASE IN HONEY

Marin Pešić (2014)
Sveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
Metadata
TitleDirektno potenciometrijsko određivanje dijastaze u medu
AuthorMarin Pešić
Mentor(s)Nikola Sakač (thesis advisor)
Abstract
Med je slatki, gusti sok što ga proizvode pčele medarice od nektara kojeg sakupljaju na cvjetovima ili sakupljanjem slatkih izlučevina (medene rose) nekih kukaca. Med sadrži amilolitički enzim dijastazu. Aktivnost dijastaza u direktom je odnosu sa starosti, kvalitetom, skladištenjem i termičkim tretmanom meda. U dijastaze spadaju enzimi -,- i - amilaze. One hidroliziraju škrob. Škrob stvara kompleks s trijodidnim ionom. Trijodidni ion čini redoks pufer trijodida i jodidnog iona. Uslijed hidrolize škroba dijastaznim enzimima iz meda mijenja se koncentracija trijodidnog iona u omjeru koncentracija redoks para trijodid/jodid, ta promjena uzrokuje promjenu potencijala koji se može mjeriti platinskim redoks senzorom. Ta promjena u omjeru redoks para trijodid/jodid opisana je Nernstovim izrazom. Povećanje potencijala kao posljedica povećanja koncentracije trijodidnog iona u omjeru koncentracija redoks para trijodid/jodid u direktnoj je korelaciji s koncentracijom dijastaze u medu. U radu je uporabom platinskog redoks senzora provedena direktna potenciometrijska metoda. Paralelno s njom izmjerena je aktivnost dijastaze uz pomoć Schade metode (standardnim postupkom). Rezultati su uspoređeni te pokazuju vrlo visok stupanj slaganja.
Keywordsdirect potentiometry diastase honey strach triiodide
Parallel title (English)DIRECT POTENTIOMETRIC DETERMINATION OF DIASTASE IN HONEY
GranterSveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineNATURAL SCIENCES
Biology
Study programme typeuniversity
Study levelgraduate
Study programmeMaster of Science Graduate University Study Programme in Biology and Chemistry; specializations in: Master Education In Chemistry and Biology
Study specializationMaster Education In Chemistry and Biology
Academic title abbreviationmag.educ.biol.et chem.
Genremaster's thesis
Language Croatian
Defense date2014-07-17
Parallel abstract (English)
Honey is a sweet, thick juice produced by the honeybees from the nectar which they collect from fresh flowers or by collecting sweet secretion (honeydew) of some insects. Honey contains amylolytic enzyme diastase. Diastasis activity is directly connected with the age, quality, storage and heat treatment of honey. The diastase enzymes are - , - and amylase which hydrolyze the starch. Starch and three-iodide make a complex, in which three-iodine ion forms redox buffer and iodide ions. Due to the hydrolysis of starch with diastasis enzymes from honey, the concentration of ions in three-iodine is changed in the ratio of concentration redox couple three - iodide / iodide. This change causes a change in the redox potential that can be measured with platinum redox sensor, since this change in ratio of redox couple three iodide / iodide is described by the Nernst expression. Potential increase, as a result of increasing the concentration of ions in three-iodide ion in ratio of concentrations in redox couple three-iodide/ iodide is in direct correlation with the concentration of diastase in honey. In this paper, we used a platinum redox sensor and direct potentiometric method. Additionally, the diastase activity was measured by Schade method (standard procedure) and the results were compared. Results showed good correlation.
Parallel keywords (Croatian)direktna potenciometrija dijastaza med škrob trijodid
Resource typetext
Access conditionNo public access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:181:024694
CommitterVesna Radman Meic