master's thesis
Influence of ß-cryptogein transgene on catalase and guaiacol-peroxidase activity in Coleus blumei hairy roots

Lavinija Mataković (2012)
Sveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
Metadata
TitleUčinak transgena za ß-kriptogein na aktivnost katalaze i gvajakol-peroksidaze ukosmatom korijenju ukrasne koprive (Coleus blumei Benth.)
AuthorLavinija Mataković
Mentor(s)Elizabeta Has Schon (thesis advisor)
Abstract
Svojstvo biotičkih elicitora da potiču obrambene odgovore u biljkama koristi se u in vitro kulturi kao strategija za povećanu proizvodnju komercijalno važnih spojeva. Elicitori se također koriste i u svrhu poboljšanja otpornosti biljke na patogene i neke okolišne stresove. Beta-kriptogein je proteinski elicitor koji izlučuje fitopatogena oomiceta Phythophthora cryptogea, koji kod biljaka izaziva hipersenzitivni odgovor i sustavno stjecanje otpornosti protiv nekih patogena. Cilj ovog istraživanja je kontroliranom sintezom kriptogeina u biljci oponašati napad patogenom, te na taj način poboljšati antioksidativni status biljke. U tu svrhu je transgeno korijenje ukrasne koprive (Coleus blumei), u kojem se sintetčki gen crypt nalazi pod kontrolom inducibilnog alkoholnog promotora, tretirano etanolom kako bi se inducirala sinteza ß-kriptogeina. Kao pokazatelji oksidativnog stresa mjerene su količine vodikova peroksida (H2O2) i produkata lipidne peroksidacije (TBARS). Antioksidativni staus je određen mjerenjem aktivnosti katalaze i gvajakol-peroksidaze. Inducibilna ekspresiju gena crypt je povećala aktivnosti antioksidativnih enzima (katalaze i gvajakol-peroksidaze) u kosmatom korijenju ukrasne koprive. Razine H2O2 i TBARS-a su pokazale odusutnost oksidativnog stresa. Povećani antioksidativni status mogao bi poboljšati otpornost biljaka protiv patogena i drugih okolišnih stresova.
KeywordsColeus blumei ß -cryptogein hairy roots oxidative stress catalase guaiacol peroxidase
Parallel title (English)Influence of ß-cryptogein transgene on catalase and guaiacol-peroxidase activity in Coleus blumei hairy roots
Committee MembersElizabeta Has Schon (committee chairperson)
Ljiljana Krstin (committee member)
Ivna Štolfa Čamagajevac (committee member)
Janja Horvatić (committee member)
GranterSveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineNATURAL SCIENCES
Biology
Study programme typeuniversity
Study levelgraduate
Study programmeMaster of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Study specializationMaster of Biology
Academic title abbreviationmag.biol.
Genremaster's thesis
Language Croatian
Defense date2012-09-28
Parallel abstract (English)
The property of biotic elicitors to induce plant defence response was used as an strategy for enhancing production of commercially important compounds in plant in vitro cultures. Also, elicitors have been used to improve plant resistance to pathogens and to some environmental stresses. Proteinaceus elicitor secreted by phytophatogenic oomycete Phythophthora cryptogea, ß-cryptogein, induces hypersensitive response and systemic acquired resistance in plants against some pathogens. The aim of this thesis was to mimic pathogen attack by using controlled synthesis of cryptogein, and thus improve antioxidant status of the plant. In order toinduce synthesis of the ß-cryptogein Coleus blumei hairy roots harboring synthetic crypt gene were generated under the control of alcohol-inducible promoter after ethanol treatment. As an indicator of the oxidative stress hydrogen peroxide (H2O2) and lipid peroxidation product (TBARS) contents were estimated. Antioxidative status was determined by measuring catalase and guiacol peroxidase activities. Induced expression of crypt gene caused increase of antioxidant enzymes activities (GPOD and CAT) in Coleus blumei hairy roots. Contents ofH2O2 and TBARS confirmed the absence of oxidative stress. Increased antioxidative status could provide enhance resistance against pathogens and other environmental stresses.
Parallel keywords (Croatian)Coleus blumei ß-kriptogein kosmato korijenje oksidacijski stres katalaza gvajakolperoksidaza
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:181:452114
CommitterVesna Radman Meic