No public access
master's thesis
Comparative proteomic analysis of biofilm forming Staphylococcis aureus spp.

Tea Kocijan (2016)
Sveučilište u Rijeci
Odjel za biotehnologiju
Metadata
TitleKomparativna proteomska analiza Staphylococcus aureus spp. sa svojstvom formiranja biofilma
AuthorTea Kocijan
Mentor(s)Alessio Soggiu (thesis advisor)
Abstract
Staphylococcus aureus je ljudski i životinjski oportunistički pathogen koji je uzrok velikog broja bolesti, uključujući trovanje hranom. Jedan od mogućih načina diseminacije ove bakterije je putem hrane kontaminirane u procesu obrađivanja od strane zaražene osobe. Također, poznato je da S. aureus formira biofilm na različitim materijalima koji se upotrebljavaju u obradi hrane i rezistentan je na većinu dezinfekcijskih sredstava. Cilj ovog rada je istražiti mehanizme formiranja biofilma zajedničke cijeloj vrsti S. aureus uspoređujući proteinsku ekspresiju planktonskih i sesilnih oblika ove bakterije. U ovom radu upotrebljen je proteomski pristup istraživanju proteina uključenih u mehanizam formiranja biofilma koji su zajednički cijeloj vrsti S. aureus. Za separaciju proteina uzoraka i identifikaciju diferencijalno eksprimiranih proteina između planktonskih i sesilnih formi ove bakterije korištena je metodologija bazirana na dvodimenzionalnoj gel elektroforezi u kombinaciji sa MALDI-TOF masenom spektrometrijom. Ukupno šest sojeva bakterije je analizirano u dva eksperimenta. Sojevi su se međusobno razlikovali u kapacitetu formiranja biofilma budući da je to doprinjelo identificiranju mehanizma specifičnog za cijelu vrstu. U dva eksperimenta je pronađeno sedam proteina diferencijalno eksprimiranih između planktonskih i sesilnih formi bakterije S. aureus koji su zatim identificirani MALDI-TOF MS tehnikom. Među identificiranim proteinima, pojačanu ekspresiju u sesilnim stanicama imali su fosfofruktokinaza, alkohol dehidrogenaza, dugolančana-masna-kiaselina-CoA-ligaza, univerzalni stres protein i protuprijenosna podjedinica mnhE2, dok su fosforibozilglicinamid formiltransferaza i acetat kinaza bili pojačano eksprimirani u planktonsim stanicama. Ovi rezultati upućuju na činjenicu da bi mehanizam formiranja biofilma mogao biti povezan se pojačanom aktivnošću glikolize i sinteze masnih kiselina, te smanjenom sintezom purina.
KeywordsStaphylococcus aureus resistance to antimicrobials biofilm formation proteomics food safety
Parallel title (English)Comparative proteomic analysis of biofilm forming Staphylococcis aureus spp.
Committee MembersAlessio Soggiu (committee chairperson)
Mirela Sedić (committee member)
Đuro Josić (committee member)
GranterSveučilište u Rijeci
Lower level organizational unitsOdjel za biotehnologiju
PlaceRijeka
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Biotechnology
Study programme typeuniversity
Study levelgraduate
Study programmeBiotechnology in medicine
Academic title abbreviationmag. biotech. in med.
Genremaster's thesis
Language Croatian
Defense date2016-07-28
Parallel abstract (English)
Staphylococcus aureus is an opportunistic human and animal pathogen that causes a wide range of diseases, including food poisoning. S. aureus is known to form biofilm on various materials used in food processing and exhibits resistance to many commonly used antimicrobials. The aim of this work is to investigate mechanisms of biofilm formation representative for the whole S. aureus specie by identifying proteins differentially expressed in planktonic and sessile forms. In this work, proteomic approach was applied to study proteins involved in species specific biofilm forming mechanism of S. aureus. Differential protein expression between planktonic and sessile forms of the bacterium was investigated using combined 2D electrophoretic separation and MALDI-TOF MS identification of designated spots. Six strains in total were used for the analysis. The strains differed in their biofilm forming capacity as the detection of common mechanisms between differently efficient biofilm producers contributed to avoiding strain specificity. Seven proteins were found to be expressed differentially between sessile and planktonic cells in the two experiments. Proteins found upregulated in the sessile cells in both experiments were ATP-dependent 6-phosphofructokinase, alcohol dehydrogenase, putative long chain-fatty acid CoA ligase, putative universal stress protein and putative antiporter subunit mnhE2, whereas phosphorybosylglycinamide formyltransferase and acetate kinase were consistently downregulated. These results indicate that the biofilm forming mechanism may be dependent on an upregulation of glucose and lipid metabolism pathways, and reduction in purine biosynthesis.
Parallel keywords (Croatian)Staphylococcus aureus rezistencija na tretman antimikrobnim sredstvima formiranje biofilma proteomska analiza sigurnost hrane
Resource typetext
Access conditionNo public access
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:193:417742
CommitterLea Lazzarich