Title Tripiridilporfirini u fotodinamičkoj inaktivaciji legionela Title (english) Tripyridylporphyrins in photodynamic inactivation of legionellae Author Josip Peradinović Mentor Nela Malatesti (mentor)Mentor Ivana Gobin (mentor)Committee member Željka Maglica (predsjednik povjerenstva)Committee member Rozi Andretić Waldowski (član povjerenstva)Committee member Nela Malatesti (član povjerenstva)Committee member Ivana Gobin (član povjerenstva)Granter University of Rijeka Defense date and country 2018-09-19, Croatia Scientific / art field, BIOTECHNICAL SCIENCES Abstract Gram-negativna bakterija Legionella pneumophila je sveprisutna u vodenim i vlažnim područjima. Zbog njene rezistentnosti na određene skupine protumikrobnih lijekova, nužni su novi pristupi u eradiciranju ove bakterije. U ovom radu istražila se mogućnost primjene fotodinamičke inaktivacije u dezinfekciji vode koja bi mogla imati primjenu u budućnosti kako bi se onemogućilo širenje zaraze uzrokovane navedenom bakterijom. Fotodinamička inaktivacija je novi pristup u eradikaciji bakterija te zahtijeva prisustvo fotosenzibilizatora, molekularnog kisika te svjetlosti. Izlaganje molekule fotosenzibilizatora svjetlosti uzrokuje nastanak reaktivnih kisikovih vrsta ili singletnog kisika koji uzrokuje smrt bakterijske stanice. Kao fotosenzibilizator u ovom radu se koristio amfifilni porfirin, 5-(4-oktadec-9-enamidofenil)-10,15,20-tris(3-piridil)porfirin. Određena je minimalna efektivna koncentracija dilucijskom metodom koja se koristila u testu fotoinaktivacije. Svi eksperimenti provedeni su u sterilnoj vodi iz slavine te se koristila ljubičasta svjetlost intenziteta 40 mW/cm2. U istraživanju se pokazalo kako je ispitivani spoj djelotvorniji nakon svjetlosne aktivacije te da ima učinak protiv stvaranja biofilma. Također se provelo ispitivanje djelovanja otopine spoja 2,5 mjeseca nakon pripreme.
U istraživanju su određene vrijednosti koncentracija MEKs = 0,391 μM, MEK2,5 = 0,195 μM. Nakon izvođenja testa toksičnosti u mraku određene su vrijednosti MEKs = 6,25 μM te MEK2,5 = 25 μM. Koncentracije koje inhibiraju adheziju iznosile su MEK = 0,391 μM nakon izlaganja svjetlosti te MEK = 0,781 μM bez izlaganja svjetlosti.
Abstract (english) Gram-negative bacterium Legionella pneumophila is ubiquitous in water and moist areas. Because of its resistance to certain types of antimicrobial drugs, the new ways of eradication are needed. The possibility of application of photodynamic inactivation in water disinfection, which may have a potential application to arrest the spreading of infection caused by aforementioned bacterium, will be presented in this work. Photodynamic inactivation is a new approach in eradication of bacteria and requires the presence of photosensitizer, molecular oxygen and light. Exposure of photosensitizer to light results in generation of reactive oxygen species or singlet oxygen which cause the death of bacterial cell. Amphiphilic porphyrin, 5-(4-octadec-9-enamidophenyl)-10,15,20-tris(3-pyridyl)porphyrin, was used as a photosensitizer in this work. Minimal effective concentration has been determined by microdillution method and it was used in the photoinactivation test. All experiments were conducted in tap water and violet light with 40 mW/cm2 fluenece was used. The tested compound was shown to be more effective after the light activation and also has an effect against the production of biofilms. An examination of the effect of the solution 2,5 months after the preparation was also conducted.
Concentrations which are determined in this research include MECs = 0,391 μM, MEC2,5 = 0,195 μM. After the dark toxicity test concentrations valued MECs = 6,25 μM and MEC2,5 = 25 μM. Concentrations which inhibits adhesion valued MEC = 0,391 μM after exposure to light and MEC = 0,781 μM after dark toxicity test.
Keywords
Legionella pneumophila
rezistencija na protumikrobne lijekove
fotodinamička inaktivacija
amfifilni porfirin
dezinfekcija vode
Keywords (english)
Legionella pneumophila
antimicrobial drug resistance
photodynamic inactivation
amphiphilic porphyrin
water disinfection
Language croatian URN:NBN urn:nbn:hr:193:428714 Study programme Title: Biotechnology and drug research Type of resource Text File origin Born digital Access conditions Open access Terms of use Repository Repository of the University of Rijeka, Department of Biotechnology Created on 2018-11-06 07:44:46
