No public access
master's thesis
Glucosylceramide critically contributes to the host defence of cystic fibrosis lungs

Barbara Kovačić (2016)
Sveučilište u Rijeci
Odjel za biotehnologiju
Metadata
TitleGlukozamid ima značajnu ulogu u imunološkoj obrani pluća oboljelih od cistične fibroze
AuthorBarbara Kovačić
Mentor(s)Erich Gulbins (thesis advisor)
Abstract
Cistična fibroza (CF) je najčešća autosomna recesivna bolest u zapadnim zemljama. Kronične upale i povećana podložnost infekcijama s patogenima poput Pseudomonas aeruginosa su glavne karakteristike cistične fibroze. Pokazano je da ceramid ima centralnu ulogu u različitim plućnim infekcijama, uključujući infekcije s Pseudomonas aeruginosa, te da se nakuplja u plućnom tkivu i stanicama oboljelih od cistične fibroze. Nadalje, ekspresija sfingozina je značajno reducirana u dišnim putevima miševa i ljudi oboljelih od cistične fibroze. No, o ulozi glukozilceramida u cističnoj fibrozi jako se malo zna. U ovom radu pokazujem da su razine glukozilceramida reducirane na apikalnoj površini bronhijalnih stanica i stanica dušnika u CF mišu. Inhalacija CF miševa s glukozilceramidom je zaštitila miševe od infekcije s Pseudomonas aeruginosa dok su ne-inhalirani CF miševi razvili tešku upalu pluća. In vitro studije nisu pokazale direktan učinak glukozilceramida na Pseudomonas aeruginosa. Ovi rezultati indiciraju da glukozilceramid ima potencijal postati nova meta u liječenju cistične fibroze.
KeywordsGlucosylceramide sphingolipids cystic fibrosis pneumonia Pseudomonas aeruginosa
Parallel title (English)Glucosylceramide critically contributes to the host defence of cystic fibrosis lungs
Committee MembersAntonija Jurak Begonja (committee chairperson)
GranterSveučilište u Rijeci
Lower level organizational unitsOdjel za biotehnologiju
PlaceRijeka
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Biotechnology
Study programme typeuniversity
Study levelgraduate
Study programmeBiotechnology in medicine
Academic title abbreviationmag. biotech. in med.
Genremaster's thesis
Language Croatian
Defense date2016
Parallel abstract (English)
Cystic fibrosis (CF) is the most common autosomal recessive disorder in Western countries and it is characterized by chronic inflammation and increased susceptibility to infection by pathogens such as Pseudomonas aeruginosa. It has been previously shown that ceramide has a central role in various pulmonary infections, including infections with P. aeruginosa, and is accumulated in lung tissues and cells from CF patients. In addition, sphingosine is severely down-regulated in the airways of CF mice and patients. However, little is known about a potential role of glucosylceramide in cystic fibrosis. Here I demonstrate that levels of glucosylceramide are reduced on the apical surface of bronchial and tracheal epithelial cells in CF mice. Inhalation of CF mice with glucosylceramide protected these mice from infection with P. aeruginosa, while non-inhaled CF mice developed severe pneumonia. However, in vitro studies failed to show a direct effect of glucosylceramide on Pseudomonas aeruginosa. This data indicate that glucosylceramide has a potential as a novel target to treat CF.
Parallel keywords (Croatian)Glukozilceramid sfingolipidi cistična fibroza upala pluća Pseudomonas aeruginosa
Resource typetext
Access conditionNo public access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:193:827447
CommitterLea Lazzarich