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master's thesis
PROIZVODNJA I KARAKTERIZACIJA MIŠJEG MONOKLONSKOG PROTUTIJELA PROTIV RIBOSOMSKOG PROTEINA S8
Rijeka: University of Rijeka, Faculty of Health Studies, 2018. urn:nbn:hr:184:194733
Matušić, Ivana
University of Rijeka
Faculty of Health Studies
Department of Basic Medical Sciences

Cite this document

Matušić, I. (2018). PROIZVODNJA I KARAKTERIZACIJA MIŠJEG MONOKLONSKOG PROTUTIJELA PROTIV RIBOSOMSKOG PROTEINA S8 (Master's thesis). Rijeka: University of Rijeka, Faculty of Health Studies. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:194733

Matušić, Ivana. "PROIZVODNJA I KARAKTERIZACIJA MIŠJEG MONOKLONSKOG PROTUTIJELA PROTIV RIBOSOMSKOG PROTEINA S8." Master's thesis, University of Rijeka, Faculty of Health Studies, 2018. https://urn.nsk.hr/urn:nbn:hr:184:194733

Matušić, Ivana. "PROIZVODNJA I KARAKTERIZACIJA MIŠJEG MONOKLONSKOG PROTUTIJELA PROTIV RIBOSOMSKOG PROTEINA S8." Master's thesis, University of Rijeka, Faculty of Health Studies, 2018. https://urn.nsk.hr/urn:nbn:hr:184:194733

Matušić, I. (2018). 'PROIZVODNJA I KARAKTERIZACIJA MIŠJEG MONOKLONSKOG PROTUTIJELA PROTIV RIBOSOMSKOG PROTEINA S8', Master's thesis, University of Rijeka, Faculty of Health Studies, accessed 26 April 2024, https://urn.nsk.hr/urn:nbn:hr:184:194733

Matušić I. PROIZVODNJA I KARAKTERIZACIJA MIŠJEG MONOKLONSKOG PROTUTIJELA PROTIV RIBOSOMSKOG PROTEINA S8 [Master's thesis]. Rijeka: University of Rijeka, Faculty of Health Studies; 2018 [cited 2024 April 26] Available at: https://urn.nsk.hr/urn:nbn:hr:184:194733

I. Matušić, "PROIZVODNJA I KARAKTERIZACIJA MIŠJEG MONOKLONSKOG PROTUTIJELA PROTIV RIBOSOMSKOG PROTEINA S8", Master's thesis, University of Rijeka, Faculty of Health Studies, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:184:194733

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Metadata
TitlePROIZVODNJA I KARAKTERIZACIJA MIŠJEG MONOKLONSKOG PROTUTIJELA PROTIV RIBOSOMSKOG PROTEINA S8
Title (english)PRODUCTION AND CHARACTERIZATION OF MOUSE MONOCLONAL ANTIBODY AGAINST RIBOSOMAL PROTEIN S8
AuthorIvana Matušić
MentorSlađana Bursać (mentor)
Committee memberLara Batičić Pučar (predsjednik povjerenstva)
Committee memberSiniša Volarević (član povjerenstva)
Committee memberSlađana Bursać (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Health Studies
(Department of Basic Medical Sciences)
Rijeka
Defense date and country2018-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Molecular Biotechnology
Abstract
Ribosomi su stanični strojevi za sintezu proteina. Eukariotski ribosom (80S) sastavljen je od male i velike podjedinice. Mala podjedinica (40S) građena je od 18S rRNK te 33 proteina, a velika podjedinica (60S) od 28S, 5,8S i 5S rRNK te 47 proteina. Ribosomski protein S8 (RPS8) sastavni je dio male podjedinice ribosoma. Cilj ovog rada bio je proizvesti mišje monoklonsko protutijelo protiv RPS8. Monoklonska protutijela prepoznaju isti epitop istog antigena. Protutijelo je proizvedeno imunizacijom miševa s rekombinantnim RPS8 sintetiziranim u bakterijama i pročišćenim afinitetnom kromatogafijom. Osim toga, slijedeći cilj bio je karakterizirati proizvedeno monoklonsko protutijela protiv RPS8 te ispitati njegovu pimjenjivost u ELISA analizi, Western blot analizi, metodi imunotaloženja te metodi imunofluorescencije. Dobiveni rezultati pokazuju da poizvedeno monoklonsko protutijelo protiv RPS8 specifično prepoznaje protein RPS8 u ELISA analizi, Western blot analizi te metodama imunotaloženja i imunofluorescencije. Protutijela imaju veliku primjenu u biomedicinskim istraživanjima te je od velike važnosti imati kvalitetna protutijela koja specifično prepoznaju protein od interesa.
Abstract (english)
Ribosomes are large molecular machines that function in protein synthesis. Eukaryotic ribosome (80S) consists of two subunits: the large and small subunits. The large subunit (60S) contains three rRNAs (28S, 5.8S and 5S) complexed with 46 proteins. The small subunit (40S) contains one rRNA (18S) complexed with 33 proteins.Ribosomal protein S8 (RPS8) is a component of a small (40S) ribosomal subunit. The goal of this work was to produce a mouse monoclonal antibody against RPS8. Monoclonal antibodies recognize the same epitope of the same antigen. The antibody was produced by immunization of mice with an recombinant RPS8 synthesized in bacteria and purified by affinity chromatography. Additionaly, the goal of this work was to characterize the mouse monoclonal antibody against the RPS8 and tested its applicability in ELISA analysis, Western blot analysis, immunoprecipitation and immunofluorescence analysis. The results show that the mouse monoclonal antibody against RPS8 specifically recognizes RPS8 protein in ELISA analysis, Western blot analysis, immunoprecipitation and immunofluorescence methods. Antibodies have many applications in biomedical research and it is important to have high quality antibodies that specifically recognize protein of interest.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:194733
Study programmeTitle: Graduate university study of medical laboratory diagnostics (Biomedicine and Healthcare; clinical medical sciences)
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the University of Rijeka, Faculty of Health Studies
Created on2018-11-20 13:36:37

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