Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom

Šuman, Frane

prikaz prve stranice dokumenta Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom

master's thesis

Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom

diplomski rad

Split: University of Split, Faculty of Chemistry and Technology, 2017. urn:nbn:hr:167:697922

Šuman, Frane

University of Split
Faculty of Chemistry and Technology
University of Split
School of Medicine

Cite this document

APA 6th Edition

Šuman, F. (2017). Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:697922

MLA 8th Edition

Šuman, Frane. "Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:697922

Chicago 17th Edition

Harvard

Šuman, F. (2017). 'Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 25 April 2024, https://urn.nsk.hr/urn:nbn:hr:167:697922

Vancouver

Šuman F. Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2017 [cited 2024 April 25] Available at: https://urn.nsk.hr/urn:nbn:hr:167:697922

IEEE

F. Šuman, "Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:167:697922

Cite this item: https://urn.nsk.hr/urn:nbn:hr:167:697922

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Metadata

Title	Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad
Title (english)	Cytotoxic activity of oleuropein extracted from wild olive leaf in various human cancer cells lines measured by MTT method : diploma thesis
Author	Frane Šuman
Mentor	Vedrana Čikeš Čulić (mentor)
Committee member	Vedrana Čikeš Čulić (predsjednik povjerenstva)
Granter	University of Split Faculty of Chemistry and Technology Split
Granter	University of Split School of Medicine Split
Defense date and country	2017-03-29, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy
Abstract	Cilj istraživanja bio je ispitati citotoksično djelovanje divlje masline (Olea europaea L. subsp. oleaster), tj. njenog glavnog fenolnog sekoiridoida, oleuropeina. Otopinom oleuropeina koncentracija 0,5 g/L i 1 g/L tretirane su humane stanice karcinoma mokraćnog mjehura (stanična linija T24), dojke (stanična linija MDA- MB-231) i pluća (stanična linija A-549). Citiotoksičnost se određivala nakon 4, 24, 48, i 72 sata MTT metodom. Usporedba apsorbancije nastalog formazana kod tretiranih i netretiranih stanica (kontrola) pokazatelj je postotka preživljenja stanica. In vitro izlaganje karcinomskih stanica oleuropeinu dovelo je do željenog smanjenog preživljenja tih stanica. Citotoksično djelovanje nije jednako na sve ispitivane karcinomske linije, a ovisno je i o koncentraciji otopine oleuropeina te vremenu izlaganja stanica. Smanjeno preživljenje nije uvijek razmjerno s vremenom izlaganja, ali je uočena povezanost s povećanjem koncentracije. Sljedeći korak su in vivo istraživanja na pokusnim životinjama.
Abstract (english)	The aim of this study was to examine citotoxic effect of wild olive (Olea europaea L. subsp. oleaster) and its main phenolic secoiridoid, oleuropein. Urinary bladder cancer cell line T24, breast cancer cell line MDAMB-231 and lung cancer cell line A-549 were treated with 0.5 g/L and 1 g/L oleuropein solution. Cytotoxicity was determined after 4, 24, 48 and 72 hours by MTT test. Percentage of cell survival was determined by comparing the absorbance of formazan in treated and untreated cells (control). In vitro exposure of cancer cells to oleuropein led to aimed cell survival reduction. The reduced survival rate was not equal for every tested cancer line and it depended on the concentration of oleuropein solution and the cell exposure time. Decreased survival was not always proportional to the exposure time, but it was proportional to concentration increase. The next step are in vivo studies on experimental animals.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:167:697922
Study programme	Title: Pharmacy Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Public note	Ključne riječi doradio knjižničar/ Key words are revised by the librarian
Repository	Repository of the Faculty of chemistry and technology
Created on	2017-07-10 09:48:40

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