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master's thesis
Razvoj metode za određivanje metadona i metabolita EDDP-a primjenom HPLC metode
diplomski rad
Split: University of Split, Faculty of Chemistry and Technology, 2017. urn:nbn:hr:167:996712
Ružić, Petra
University of Split
Faculty of Chemistry and Technology
University of Split
School of Medicine

Cite this document

Ružić, P. (2017). Razvoj metode za određivanje metadona i metabolita EDDP-a primjenom HPLC metode : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:996712

Ružić, Petra. "Razvoj metode za određivanje metadona i metabolita EDDP-a primjenom HPLC metode : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:996712

Ružić, Petra. "Razvoj metode za određivanje metadona i metabolita EDDP-a primjenom HPLC metode : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:996712

Ružić, P. (2017). 'Razvoj metode za određivanje metadona i metabolita EDDP-a primjenom HPLC metode : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 16 April 2024, https://urn.nsk.hr/urn:nbn:hr:167:996712

Ružić P. Razvoj metode za određivanje metadona i metabolita EDDP-a primjenom HPLC metode : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2017 [cited 2024 April 16] Available at: https://urn.nsk.hr/urn:nbn:hr:167:996712

P. Ružić, "Razvoj metode za određivanje metadona i metabolita EDDP-a primjenom HPLC metode : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:167:996712

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Metadata
TitleRazvoj metode za određivanje metadona i metabolita EDDP-a primjenom HPLC metode : diplomski rad
Title (english)Development of a HPLC method for the determination of methadone and its metabolite EDDP : diploma thesis
AuthorPetra Ružić
MentorDavorka Sutlović (mentor)
Committee memberVedrana Čikeš Čulić (predsjednik povjerenstva)
Committee memberMarija Definis-Gojanović (član povjerenstva)
Committee memberDavorka Sutlović (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
Split
GranterUniversity of Split
School of Medicine
Split
Defense date and country2017-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Abstract
Ciljevi istraživanja: Razviti metodu za kvalitativno i kvantitativno određivanje metadona i EDDP-a u biološkim uzorcima korištenjem HPLC tehnike, koja se može primijeniti u stvarnoj praksi određivanja nepoznate koncentracije metadona u pacijenata na supstitucijskoj terapiji metadonom. Materijali i metode: Metodom HPLC su analizirane standardne otopine metadona i EDDP-a, pripremljene razrjeđivanjem standarda metadona i EDDP-a (Lipomed) te otopine Heptanona u metanolu. Nakon što su metadon i EDDP detektirani u standardnim otopinama te nakon ekstrakcije oba analita iz seruma korištenjem tehnike LLE (engl. liquid-liquid extraction), ekstrahirani analiti (smjesa metadona i EDDP-a) su u koncentraciji od 1 mg/L i volumenu od 10 µL injektirani u aparat. Optimalna valna duljina je bila 220 nm, kao pokretna faza je korištena smjesa metanola, acetonitrila i vode (v/v/v=9:11:80), pH= 6.0, temperatura sustava je namještena na 30°C, a vrijeme trajanja analize iznosilo je 8 minuta. Protok je bio namješten na 0.6 mL/min do četvrte minute pa na 0.4 mL/min od četvrte do osme minute. Rezultati: Injektiranjem standardne otopine metadona u prethodno navedenim uvjetima, kao i standardne otopine EDDP-a, oba su spoja na instrumentu detektirana oko šeste minute. Ipak, analiti ekstrahirani iz seruma (smjesa metadona i EDDP-a) su u istim uvjetima na kromatogramu detektirani kao jedan pik, umjesto dva odvojena pika koja su trebala odgovarati dvjema različitim tvarima. Zaključci: Analizom u prethodno obrazloženim uvjetima metadon i EDDP su kvalitativno dokazani, tj. dokazano je njihovo prisustvo u serumu. Ipak, navedenom metodom nije ih moguće dokazati kvantitativno, što znači da se ona ne može primijeniti u stvarnoj praksi određivanja nepoznate koncentracije lijeka i njegovih metabolita u biološkom uzorku.
Abstract (english)
Objectives: The aim of this study was to develop an analytical method for the HPLC determination of methadone and EDDP in biological fluids, which could be readily applied to the monitoring of methadone levels in biological fluids of patients undergoing methadone maintenance therapy. Materials and methods: Methadone and EDDP standard solutions were preapared after diluting the Heptanon solution, and both methadone and EDDP reference standards from Lipomed in methanol and then injected in the HPLC. After the methadone and EDDP standard solutions were detected on the instrument and both of the analites were extracted from serum by LLE, 10 µL of the extracted substances (the mixture of methadone and EDDP) was injected in the concetration 1 mg/L. The chromatografic procedure was carried out by setting a detection wavelength at 220 nm, the working temperature at 30°C and using mixture of methanol, acetonitrile and water (v/v/v=9:11:80), pH= 6.0, as mobile phase. The analysis was performed for 8 minutes total. The flow rate of the mobile phase was 0.6 mL/min during the first four minutes and then switched to 0.4 mL/min from the fourth minute to the end of the analysis. Results: Both of methadone and EDDP standard solutions were eluted around sixth minute. Still, the mixture of methadone and EDDP extracted from the serum was detected as only one peak on the chromatogram, instead of two separate peaks which should have correspond to these two different substances. Conclusion: Using the HPLC method described herein, the presence of both methadone and EDDP in the serum could be detected. However, their concentration could not be quantified, therefore this method could not be used for the determination of a drug and its metabolites in biological fluids.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:996712
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteKljučne riječi doradio knjižničar/ Key words are revised by the librarian
RepositoryRepository of the Faculty of chemistry and technology
Created on2018-07-20 11:00:06

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