UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU

Vragović, Martina

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undergraduate thesis

UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU

Rijeka: University of Rijeka, Faculty of Medicine, 2018. urn:nbn:hr:184:547675

Vragović, Martina

University of Rijeka
Faculty of Medicine
Department of Chemistry and Biochemistry

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APA 6th Edition

Vragović, M. (2018). UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU (Undergraduate thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:547675

MLA 8th Edition

Vragović, Martina. "UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU." Undergraduate thesis, University of Rijeka, Faculty of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:184:547675

Chicago 17th Edition

Harvard

Vragović, M. (2018). 'UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU', Undergraduate thesis, University of Rijeka, Faculty of Medicine, accessed 23 April 2024, https://urn.nsk.hr/urn:nbn:hr:184:547675

Vancouver

Vragović M. UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU [Undergraduate thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2018 [cited 2024 April 23] Available at: https://urn.nsk.hr/urn:nbn:hr:184:547675

IEEE

M. Vragović, "UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU", Undergraduate thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:184:547675

Cite this item: https://urn.nsk.hr/urn:nbn:hr:184:547675

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Metadata

Title	UTJECAJ pH NA DINAMIKU NOSAČA LIJEKA TEMELJENOG NA N-(2-HIDROKSIPROPIL)METAKRILAMIDU
Title (english)	INFLUENCE OF pH ON DYNAMICS OF DRUG CARRIER BASED ON N-(2-HYDROXYPROPYL)METHACRYLAMIDE
Author	Martina Vragović
Mentor	Damir Klepac (mentor)
Committee member	Dijana Detel (predsjednik povjerenstva)
Committee member	Marin Tota (član povjerenstva)
Committee member	Damir Klepac (član povjerenstva)
Granter	University of Rijeka Faculty of Medicine (Department of Chemistry and Biochemistry) Rijeka
Defense date and country	2018-09-21, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Basic Medical Sciences Medical Biochemistry
Abstract	U ovom radu ispitano je otpuštanje nitroksilnih radikala koji se koriste za liječenje bolesti uzrokovanih oksidacijskim stresom sa polimernog nosača temeljenog na poli[N-(2-hidroksipropil)metakrilamidu] (poly(HPMA)). 4-okso-2,2,6,6-tetrametilpiperidin-1-oksil (TEMPONE) radikali vezani su na poly(HPMA) nosač preko razmaknice koja sadrži pH-osjetljivu hidrazonsku vezu. Priređeni hidrofilni polimer otopljen je u fosfatnom puferu pri različitim pH vrijednostima (7,4, 6,8, 6,0, 5,0). Hidrodinamički radijus molekula nosača u otopinama određen je metodom dinamičkog raspršenja svjetlosti (DLS). Pokazano je da su molekule poly(HPMA) u otopinama poprimile konformaciju statističkog klupka hidrodinamičkog radijusa 3,5 nm sa širokom razdiobom veličina. Oslobađanje nitroksilnih radikala sa poly(HPMA) nosača određivano je metodom elektronske spinske rezonancije (ESR). Utvrđeno je da se radikali brzo oslobađaju sa nosača pri uvjetima karakterističnim za endosome tumorskih stanica (pH = 5,0) dok pri fiziološkim uvjetima (pH = 7,4) radikali uglavnom ostaju vezani na nosaču. Na ovaj način postiže se kontrolirano otpuštanje radikala u tumoru te se izbjegavaju neželjeni učinci nitroksilnih radikala na ostale organe i tkiva.
Abstract (english)	In this thesis, the release of nitroxyl radicals used to treat diseases caused by oxidative stress from poly[N-(2-hydroxypropyl)methacrylamide] (poly(HPMA)) is investigated. 4-oxo-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPONE) radicals are attached to a poly(HPMA) carrier by a spacer containing pH-sensitive hydrazone linkage. The prepared hydrophilic polymer is dissolved in phosphate buffer at various pH values (7.4, 6.8, 6.0, 5.0). The hydrodynamic radius of the carrier molecules in solutions was determined by the dynamic light scattering method (DLS). It has been shown that the poly(HPMA) molecules in the solutions obtained random coil conformation with the hydrodynamic radius of 3.5 nm and broad size distribution. The release of nitroxyl radicals from poly(HPMA) carrier was determined by the electron spin resonance (ESR) method. It was found that the radicals are quickly released from the carrier under conditions characteristic for the endosomal tumor cells (pH = 5.0) while at physiological conditions (pH = 7.4) the radicals remained largely bound to the carrier. In this way, controlled release of the radicals in the tumor can be achieved and the unwanted effects of nitroxyl radicals on other organs and tissues are avoided.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:184:547675
Study programme	Title: Study of Sanitary Engineering Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) sanitarnog inženjerstva)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the University of Rijeka, Faculty of Medicine
Created on	2018-12-07 08:28:07

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