Imidacloprid is a pesticide used to protect the plants from insects. Usually it comes in a formulation called Confidor. Imidacloprid belongs to the group of neonicotinoids, meaning it acts agonistically on nicotine receptors in the nervous system. Although its primary mechanism of action is well known, there is a possibility that there may be some other mechanisms. Therefore, it is necessary to investigate whether there is a secondary mechanism present in imidacloprid acting, along with the impact on nicotine receptors. Model organisms such as yeast Saccharomyces cerevisiae are suitable for such studies. Yeasts are similar in many metabolic processes with the human, so the results of imidacloprid impact on the yeast cells can be transfered to the human cells. This work investigates the impact of Confidor on S. cerevisiae lipid metabolism. The yeast was treated with Confidor and the total lipids (using Folch method) and mitochondria was isolated. Furthermore, lipids were separated on fractions (neutral lipids, phospholipids, free fatty acids, sterol esters, triacylglycerols, sterols, diacylglycerols and monoacylglycerols) by solid phase extraction (SPE). The lipids of individual fractions were then methylated and analyzed by gas chromatography. The results have shown that Confidor has an impact on yeasts, reducing its vitality. Between control samples and samples of yeast treated with Confidor there are differences in the composition of fatty acids. Also, there are differences in quantity of individual lipid fractions, so that sterol, triacylglycerol and monoacylglycerol levels differ significantly between the control sample and the yeast treated with Confidor. It can be concluded that Confidor has an effect on lipids of yeast S. cerevisiae which can be seen in changes of their composition and content.