Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima

Marković, Leon

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master's thesis

Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima

Zagreb: University of Zagreb, School of Medicine, 2016. urn:nbn:hr:105:669602

Marković, Leon

University of Zagreb
School of Medicine
Department of Medical Biology

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APA 6th Edition

Marković, L. (2016). Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:669602

MLA 8th Edition

Marković, Leon. "Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima." Master's thesis, University of Zagreb, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:105:669602

Chicago 17th Edition

Harvard

Marković, L. (2016). 'Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima', Master's thesis, University of Zagreb, School of Medicine, accessed 25 April 2024, https://urn.nsk.hr/urn:nbn:hr:105:669602

Vancouver

Marković L. Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2016 [cited 2024 April 25] Available at: https://urn.nsk.hr/urn:nbn:hr:105:669602

IEEE

L. Marković, "Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:105:669602

Cite this item: https://urn.nsk.hr/urn:nbn:hr:105:669602

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Metadata

Title	Čimbenici transkripcije TCF-1 i LEF-1 uključeni u signalni put Wnt izraženi su u tumorima mozga astrocitomima
Title (english)	Transcription factors TCF-1 and LEF-1 involved in Wnt signaling in astrocyte brain tumors
Author	Leon Marković
Mentor	Nives Pećina-Šlaus (mentor)
Committee member	Davor Tomas (predsjednik povjerenstva)
Committee member	Ljiljana Šerman (član povjerenstva)
Committee member	Nives Pećina-Šlaus (član povjerenstva)
Granter	University of Zagreb School of Medicine (Department of Medical Biology) Zagreb
Defense date and country	2016-07-15, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Clinical Medical Sciences Neurology
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Clinical Medical Sciences Oncology
Abstract	Obitelj transkripcijskih faktora TCF/LEF od velikog je značaja u biologiji tumora. Predmet istraživanja u ovom radu bila je razina ekspresije TCF-1 i LEF-1, u astrocitomima različitih histopatoloških gradusa (I, II i III). Razine ekspresije TCF-1 i LEF-1 određene su metodom imunohistokemije. Utvrđena je ekspresija transkripcijskih čimbenika TCF-1 i LEF-1 lokalizirana u jezgrama stanica astrocitoma. Za TCF-1 određen je porast udjela stanica u kategorijama jake i srednje razine ekspresije, koji je u pozitivnoj korelaciji sa porastom gradusa, sa najvišim vrijednostima u astrocitomima gradusa III -jaka (4,67%), srednja (51,85%), a u njima je očekivano smanjen udio stanica niske ekspresije (43,46%). Za LEF-1 utvrđen je pad ekspresije u kategorijama srednje i niske razine ekspresije, dok je za razinu jake ekspresije određen porast ekspresije koji korelira sa porastom gradusa astrocitoma, sa najvišom vrijednošću jake ekspresije u astrocitomima III (3,73%). Prema našim rezultatima zaključujemo da je porast ekspresije TCF-1 i LEF-1 u pozitivnoj korelaciji sa porastom gradusa astrocitoma. Izraženost transkripcijskih čimbenika TCF-1 i LEF-1 upućuje na uključenost signalnog puta Wnt u nastanku i progresiji tumora mozga astrocitoma.
Abstract (english)	TCF/LEF family of transcription factors is of great significance in tumor biology. The subject of this research was to determine the levels of expression of TCF-1 and LEF-1, in astrocytoma of different histopathologic grade (I, II and III). Immunohistochemistry was the method of choice for determining the levels of expression of TCF-1 and LEF-1. The expression of transcription factors TCF-1 and LEF-1 was localised within the nuclei of astrocytic tumor cells. In case of TCF-1, there was an increase in percentage of cells in categories of strong and intermediate levels of expression, which is in positive correlation with the increasement of grade, with peak values in astrocytoma grade III -strong (4,67%), intermediate (51,85%), as expected there was decreasement in percentage of cells with low expression (43,46%). On the other hand, results for LEF-1 displayed decreased expression in categories with intermediate and low levels of expression, while for the level of strong expression was determined increase in expression which correlated with the increasement of astrocytomic grade, with the peak value in astrocytoma III (3,73). Based on our results, we conclude that the increase in expression of TCF-1 and LEF-1 is in positive correlation with the increase in astrocytoma grade. The expression of transcription factors TCF-1 and LEF-1 suggests activation of Wnt signaling pathway in formation and progression of astrocytic brain tumors.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:105:669602
Study programme	Title: Medicine Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Repository	Dr Med - University of Zagreb School of Medicine Digital Repository
Created on	2017-08-21 09:02:43

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