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undergraduate thesis
Mutabilnost bakterija mliječne kiseline Lactobacillus brevis I Lactobacillus plantarum
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. urn:nbn:hr:159:148467
Vlainić, Vedrana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Cite this document

Vlainić, V. (2010). Mutabilnost bakterija mliječne kiseline Lactobacillus brevis I Lactobacillus plantarum (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:148467

Vlainić, Vedrana. "Mutabilnost bakterija mliječne kiseline Lactobacillus brevis I Lactobacillus plantarum." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:148467

Vlainić, Vedrana. "Mutabilnost bakterija mliječne kiseline Lactobacillus brevis I Lactobacillus plantarum." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:148467

Vlainić, V. (2010). 'Mutabilnost bakterija mliječne kiseline Lactobacillus brevis I Lactobacillus plantarum', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:148467

Vlainić V. Mutabilnost bakterija mliječne kiseline Lactobacillus brevis I Lactobacillus plantarum [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2010 [cited 2024 April 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:148467

V. Vlainić, "Mutabilnost bakterija mliječne kiseline Lactobacillus brevis I Lactobacillus plantarum", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:159:148467

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Metadata
TitleMutabilnost bakterija mliječne kiseline Lactobacillus brevis I Lactobacillus plantarum
Title (english)Mutability of lactic acid bacterium Lactobacillus brevis and Lactobacillus plantarum
AuthorVedrana Vlainić
MentorVišnja Bačun-Družina (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2010-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Bakterije u svojem prirodnom okruženju pri izlaganju promjenjivim okolišnim uvjetima podliježu neprestanim promjenama genetičkog materijala. Promjene mogu nastati spontano, ili mogu biti uzrokovane adaptivnom mutagenezom, hipermutacijama ili horizontalnim prijenosom gena. Mutacije su osnovni pokretač evolucije i ključne su za preživljavanje bakterija u stresnim uvjetima. U ovom radu pratio se rast spontanih mutanata bakterija mliječne kiseline u produljenoj stacionarnoj fazi u usporedbi s bakterijama divljeg tipa. Stabilnost genetičkog materijala mutanata ispitivana je metodom gel elektroforeze u pulsirajućem polju. Dobiveni rezultati pokazuju da su mutanti stabilniji i prilagodljiviji naspram stanica divljeg tipa tijekom uzgoja u produljenoj stacionarnoj fazi rasta dok analiza genoma bakterija nije pokazala značajne razlike između mutanata i stanica divljeg tipa.
Abstract (english)
While growing in their natural enviroment, bacteria are exposed to commutable enviromental conditions causing their genetic material to change. Such alterations can be the consequence of spontaneous mutatios or caused by adaptive mutagenesis, hypermutagenesis or by horizontal gene transfer. Mutations are the basic promoter of evolution and are essential for the survival of bacteria growing in a stressful enviroment. The aim of this study was to compare the growth of spontaneous mutants of lactic acid bacteria to the growth of their respective wild type strains. The stability of the mutants' genetic material was analysed by the pulsed field gel electrophoresis method. The results show that the mutants are more stable and adjustable in comparison to the wild type strain during their growth in the prolonged stationary phase while the gel analisys didn't show any significant differences between the mutants' genome and the one belonging to the wild type bacteria.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:148467
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2017-06-07 09:52:01

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