Utjecaj egzonukleaze vii na neobuzdanu degradaciju DNA u recA mutantima bakterije Escherichia coli

Briški, Nina

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master's thesis

Utjecaj egzonukleaze vii na neobuzdanu degradaciju DNA u recA mutantima bakterije Escherichia coli

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. urn:nbn:hr:159:785450

Briški, Nina

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

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APA 6th Edition

Briški, N. (2010). Utjecaj egzonukleaze vii na neobuzdanu degradaciju DNA u recA mutantima bakterije Escherichia coli (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:785450

MLA 8th Edition

Briški, Nina. "Utjecaj egzonukleaze vii na neobuzdanu degradaciju DNA u recA mutantima bakterije Escherichia coli." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:785450

Chicago 17th Edition

Harvard

Briški, N. (2010). 'Utjecaj egzonukleaze vii na neobuzdanu degradaciju DNA u recA mutantima bakterije Escherichia coli', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:785450

Vancouver

Briški N. Utjecaj egzonukleaze vii na neobuzdanu degradaciju DNA u recA mutantima bakterije Escherichia coli [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2010 [cited 2024 April 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:785450

IEEE

N. Briški, "Utjecaj egzonukleaze vii na neobuzdanu degradaciju DNA u recA mutantima bakterije Escherichia coli", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:159:785450

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:785450

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Metadata

Title	Utjecaj egzonukleaze vii na neobuzdanu degradaciju DNA u recA mutantima bakterije Escherichia coli
Title (english)	Impact of the exonuclease vii on the reckless DNA degradation in the recA mutants in Escherichia coli bacterium
Author	Nina Briški
Mentor	Davor Zahradka (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Laboratory for Biology and Microbial Genetics) Zagreb
Defense date and country	2010-10-20, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Protein RecA ima ključnu ulogu u homolognoj rekombinaciji kod bakterije Escherichia coli. Stanice recA mutanata pokazuju značajan pad vijabilnosti, gubitak sposobnosti za rekombinaciju i sklonost neobuzdanoj degradaciji DNA. U ovom radu je proučavan utjecaj inaktivacije gena xseA (koji kodira egzonukleazu VII) na neobuzdanu degradaciju DNA u recA mutantima. Učinak mutacije xseA praćen je zasebno i u kombinaciji s mutacijama xonA i sbcD, koje inaktiviraju egzonukleazu I, odnosno nukleazu SbcCD. Degradacija DNA je praćena tijekom normalnog rasta stanica i nakon izlaganja UV-zračenju. Za praćenje degradacije DNA korištene su mikroskopske metode i elektroforeza u promjenjivom električnom polju. Rezultati su pokazali da inaktivacija egzonukleaze VII, egzonukleaze I i nukleaze SbcCD ima sinergistički učinak i dokida neobuzdanu degradaciju DNA u stanicama recA mutanata.
Abstract (english)	RecA protein has a major role in homologous recombination in Escherichia coli. Cells mutated in recA gene show decreased cell viability, homologous recombinaton deficiency and tendency for reckless DNA degradation. The aim of this work was to study the effect of the xseA mutation (that inactivates exonuclease VII) on reckless DNA degradation in recA mutants. Effect of xseA mutation was examined separately as well as in combination with xonA and sbcD mutations, which inactivate exonuclease I and SbcCD nuclease, respectively. DNA degradation was followed in normally growing as well as in UV-irradiated cells, by microscopic methods and by pulsed-field gel electrophoresis. The results have shown that inactivation of exonuclease VII, exonuclease I and SbcCD nuclease has sinergistic effect and abolishes reckless DNA degradation in recA mutant cells.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:785450
Study programme	Title: Biotechnology; specializations in: Biochemical-Microbiology Course: Biochemical-Microbiology Study programme type: university Study level: graduate Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2017-08-25 07:47:58

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