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master's thesis
Izolacija i analiza mikroorganizama iz okoliša
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:866249
Lovrić, Blanka
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Cite this document

Lovrić, B. (2015). Izolacija i analiza mikroorganizama iz okoliša (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:866249

Lovrić, Blanka. "Izolacija i analiza mikroorganizama iz okoliša." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:866249

Lovrić, Blanka. "Izolacija i analiza mikroorganizama iz okoliša." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:866249

Lovrić, B. (2015). 'Izolacija i analiza mikroorganizama iz okoliša', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 16 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:866249

Lovrić B. Izolacija i analiza mikroorganizama iz okoliša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 April 16] Available at: https://urn.nsk.hr/urn:nbn:hr:159:866249

B. Lovrić, "Izolacija i analiza mikroorganizama iz okoliša", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:866249

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Metadata
TitleIzolacija i analiza mikroorganizama iz okoliša
Title (english)Isolation and identification of microorganisms from environment
AuthorBlanka Lovrić
MentorVišnja Bačun-Družina (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2015-10-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Mnogi mikroorganizmi mogu koristiti ugljikovodike kao izvor ugljika i energije za metaboličke aktivnosti te su takvi mikroorganizmi široko rasprostranjeni u prirodi. Glavni mikrobiološki problem u naftnoj industriji su kontaminacije uskladištenih produkata odnosno goriva, koje uzrokuje gubljenje kvalitete proizvoda te stvaranja mulja što dovodi do kvarenja proizvoda, začepljena cjevovoda i tankova. Ugljikovodici iz goriva su čest uzrok onečišćenja okoliša pa na naftnoj i industriji leži velika odgovornost za rješavanje problema onečišćenja okoliša. S ciljem identifikacije mikroorganizama, u ovom radu izolirani su mikroorganizmi iz spremnika s dizelskim gorivom. Korištena je metoda identifikacije bazirana na proteomskom pristupu koja uključuje primjenu spektrometra masa MALDI/TOF-TOF. Od 9 izoliranih vrsta, biotipizacijom pomoću programskog alata Protein Reader identificirano je 5 različitih vrsta. Najzastupljenija vrsta je Nectria haematococca.
Abstract (english)
Many microorganisms can utilize hydrocarbons as sole sources of carbon as energy for metabolic activities and these microorganisms are present and widely distributed in the nature. The major microbial problem in the petroleum refining industry is contamination of stored products, which can lead to loss of product quality, formation of sludge and deterioration of pipework and storage tanks. Hydrocarbons from fuel are common cause of environmental pollution so there is a great responsibility on petroleum industry for solving the problem of hydrocarbons pollution. In order to identify microorganisms, in this work we isolated microorganisms from diesel fuel storage tank. Method used for identification is based on proteomic approach and includes application of mass spectrometry. From 9 isolated species we identified 5 different species by biotypization protocol using software Protein Reader. Most common species is Nectria haematococca.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:866249
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2017-04-27 12:13:50

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