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master's thesis
Određivanje fuzarijskih mikotoksina ELISA metodom
DON, zearalenon, fuzarijski toksini, mikotoksikoze
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:894393
Kvočić, Tonka
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Process Engineering
Section for Fundamental Engineering

Cite this document

Kvočić, T. (2015). Određivanje fuzarijskih mikotoksina ELISA metodom : DON, zearalenon, fuzarijski toksini, mikotoksikoze (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:894393

Kvočić, Tonka. "Određivanje fuzarijskih mikotoksina ELISA metodom : DON, zearalenon, fuzarijski toksini, mikotoksikoze." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:894393

Kvočić, Tonka. "Određivanje fuzarijskih mikotoksina ELISA metodom : DON, zearalenon, fuzarijski toksini, mikotoksikoze." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:894393

Kvočić, T. (2015). 'Određivanje fuzarijskih mikotoksina ELISA metodom : DON, zearalenon, fuzarijski toksini, mikotoksikoze', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 16 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:894393

Kvočić T. Određivanje fuzarijskih mikotoksina ELISA metodom : DON, zearalenon, fuzarijski toksini, mikotoksikoze [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 April 16] Available at: https://urn.nsk.hr/urn:nbn:hr:159:894393

T. Kvočić, "Određivanje fuzarijskih mikotoksina ELISA metodom : DON, zearalenon, fuzarijski toksini, mikotoksikoze", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:894393

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Metadata
TitleOdređivanje fuzarijskih mikotoksina ELISA metodom : DON, zearalenon, fuzarijski toksini, mikotoksikoze
Title (english)Determination of fusarium mycotoxins using ELISA method
AuthorTonka Kvočić
MentorKsenija Markov (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Process Engineering)
(Section for Fundamental Engineering)
Zagreb
Defense date and country2015-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Kontaminacija hrane i hrane za ţivotinje mikotoksinima značajan je svjetski problem. Mikotoksini su sekundarni metaboliti plijesni koji imaju negativne učinke na zdravlje ljudi, ţivotinja i usijeve rezultirajući bolestima i ekonomskim gubicima. Deoksinivalenol (DON) i zearalenon su mikotoksini sintetizirani od nekoliko vrsta plijesni roda Fusarium. U ovom istraţivanju,27 uzoraka kukuruza i 26 uzorka pšenice prikupljeni su upodručju istočne i sjeverozapadne Hrvatske tijekom 2014. godine. Koncentracije DON-a i zearalenona odreĎivane su primjenom validirane ELISA metode. Koncentracija DON-a premašuje najveće dopuštene vrijednosti u šest uzorka pšenice i sedam uzorka kukuruza, dok je koncentracija zearalenona veća od propisane izmjerena u tri uzorka pšenice i devet uzorka kukuruza. UsporeĎujući broj pozitivnih uzorka odnosno koncentracije analiziranih mikotoksina u pšenici i kukuruzu, kukuruz se pokazao kao više kontaminirana ţitarica. Visoke koncentracije DON-a i zearalenona u analiziranim ţitaricama mogu se povezati sa vremenskim uvjetima koji su 2014. godine okarakterizirani kao kišni do vrlo kišni te topli i vrlo topli.
Abstract (english)
The worldwide contamination of foods and feeds with mycotoxins is a significant problem. Mycotoxins are secondary metabolites of molds that have adverse effects on humans, animals and crops that result in illnesses and economic losses. Deoxynivalenol(DON) and zearalenone are mycotoxins produced by several species of mold which belong to the genus Fusarium. In this study, a total of 27 maize samples and 26 samples of wheat were collected from different fields situated in eastern and northwestern part of Croatia during 2014. Determination of DON and zearalenone concentration was done using validated ELISA (Enzyme-Linked Immunosorbent Assay) method. DON concentrations higher than allowed were observed in six wheat samples and seven maize samples while zearalenone concentration was higher than allowed in three wheat samples and nine maize samples. Comparing the number of positive samples and analyzed mycotoxin concentrations in wheat and maize, maize proved to be more contaminated cereal.High concentrations of DON and zearalenone in the analyzed cereals may be associated with weather conditions that characterized 2014 as a rainy to very rainy and warm to very warm.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:894393
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2017-05-04 10:04:26

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