prikaz prve stranice dokumenta Ispitivanje načina vezanja Scw4 proteina u staničnu stijenku kvasca Saccharomyces cerevisiae
  Download
PDF 993.79 KB
undergraduate thesis
Ispitivanje načina vezanja Scw4 proteina u staničnu stijenku kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:685729
Lulić, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Cite this document

Lulić, L. (2016). Ispitivanje načina vezanja Scw4 proteina u staničnu stijenku kvasca Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:685729

Lulić, Lucija. "Ispitivanje načina vezanja Scw4 proteina u staničnu stijenku kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:685729

Lulić, Lucija. "Ispitivanje načina vezanja Scw4 proteina u staničnu stijenku kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:685729

Lulić, L. (2016). 'Ispitivanje načina vezanja Scw4 proteina u staničnu stijenku kvasca Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:685729

Lulić L. Ispitivanje načina vezanja Scw4 proteina u staničnu stijenku kvasca Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:685729

L. Lulić, "Ispitivanje načina vezanja Scw4 proteina u staničnu stijenku kvasca Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:685729

Please login to the repository to save this object to your list.
Metadata
TitleIspitivanje načina vezanja Scw4 proteina u staničnu stijenku kvasca Saccharomyces cerevisiae
Title (english)Investigation of the binding of the Scw4 protein into the cell wall of yeast Saccharomyces cerevisiae
AuthorLucija Lulić
MentorRenata Teparić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2016-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Scw4 je protein koji se u staničnoj stijenci kvasca Saccharomyces cerevisiae veže kovalentno i nekovalentno, ali nije poznato koji dio gena kodira za regiju koja mu omogućuje kovalentno povezivanje. Provedeno je ispitivanje načina vezanja u stijenku nakon delecije dijelova gena SCW4 koji kodiraju za regije za koje se pretpostavlja da bi mogle omogućiti kovalentno povezivanje. U tu je svrhu kvasac uzgojen u selektivnoj YNB podlozi nakon čega su izolirane stijenke i njihovi proteini kuhanjem u SDS-u uz dodatak β-merkaptoetanola odnosno tretmanom stijenki s 30 mM NaOH. Izolirani proteini su zatim analizirani SDS elektroforezom po Laemmli-u i metodom Western blot. Prema dobivenim rezultatima može se pretpostaviti da se Scw4 protein u staničnu stijenku kvasca veže kovalentno preko regije koja je prema broju i redoslijedu glutaminskih ostataka slična repetitivnoj sekvenci PIR proteina. Dobivene rezultate je potrebno potvrditi daljnjim istraživanjima.
Abstract (english)
Scw4 is a protein which can be bound covalently and non-covalently into the cell wall of yeast Saccharomyces cerevisiae, but it is not known which region of protein allows its covalent linkage. The way of its binding was examined after deletion of SCW4 gene segments encoding regions that could be responsible for covalent attachment of Scw4. For this purpose, the yeast was cultivated in a selective YNB medium and after that, the cell walls and proteins were isolated by boiling in SDS with β-mercaptoethanol or by treatment with a 30 mM NaOH. Isolated proteins were examined by SDS electrophoresis according to Laemmli and by Western blotting. According to the results, it can be assumed that the protein Scw4 is bound covalently via a region that has an arrangement and number of glutamine residues similar to that found in the repetitive sequence of PIR proteins that is responsible for its covalent binding. This results should be confirmed in further experiments.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:685729
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2017-04-24 11:23:16

(©) Nacionalna i sveučilišna knjižnica u Zagrebu 2015. Sva prava pridržana. | Hrvatske bratske zajednice 4 p.p. 550, 10000 Zagreb, Hrvatska Tel. ++ 385 1 616-4040
OIB: 84838770814 | IBAN: HR66 2340 0091 1000 1008 1, Porezni broj (PDV identifikacijski broj) - VAT Number: HR84838770814
Prijava u repozitorij