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undergraduate thesis
Enzimski katalizirana hidroliza (R, S)-1-feniletil acetata u eutektičkim otapalima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:381555
Rajn, Martina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

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Rajn, M. (2017). Enzimski katalizirana hidroliza (R, S)-1-feniletil acetata u eutektičkim otapalima (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:381555

Rajn, Martina. "Enzimski katalizirana hidroliza (R, S)-1-feniletil acetata u eutektičkim otapalima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:381555

Rajn, Martina. "Enzimski katalizirana hidroliza (R, S)-1-feniletil acetata u eutektičkim otapalima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:381555

Rajn, M. (2017). 'Enzimski katalizirana hidroliza (R, S)-1-feniletil acetata u eutektičkim otapalima', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:381555

Rajn M. Enzimski katalizirana hidroliza (R, S)-1-feniletil acetata u eutektičkim otapalima [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:381555

M. Rajn, "Enzimski katalizirana hidroliza (R, S)-1-feniletil acetata u eutektičkim otapalima", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:381555

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Metadata
TitleEnzimski katalizirana hidroliza (R, S)-1-feniletil acetata u eutektičkim otapalima
Title (english)Enzymic catalytic hydrolysis (R, S)-1-phenylethyl acetate in deep eutectic solvents
AuthorMartina Rajn
MentorMarina Cvjetko Bubalo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2017-07-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Visokoučinkoviti i ekološki prihvatljivi procesi proizvodnje industrijski važnih kiralnih građevnih blokova, biokatalitičkih postupaka te postupaka u kojima se primjenjuju zelena otapala, posljednjih su godina predmet istraživanja. Cilj ovog rada bio je istražiti dvije vrste biokatalizatora, izoliranu lipazu CALB i korijen mrkve, za hidrolizu (R, S)-1-feniletil acetata u različitim eutektičkim otapalima. Obzirom na praćeno iskorištenje reakcije, enantiomerni višak, specifičnu produktivnost enzima i volumetrijsku produktivnost enzima može se zaključiti da su prirodna eutektička otapala superiornija za hidrolizu (R, S)-1-feniletil acetata s izoliranom lipazom od hlapljivih organskih otapala. Hidrolizom pomoću mrkve nema značajnijih pomaka u iskorištenju i produktivnosti u odnosu na organska otapala, no vidljivo je poboljšanje u enantioselektivnosti. Rezultati prikazani u ovom istraživanju doprinos su razvoju učinkovitih, ekonomičnih i održivih procesa za hidrolizu industrijski važnih spojeva.
Abstract (english)
Highly efficient and environmentally-acceptable processes for the production of industrially important chiral building blocks, biocatalytic processes and green solvent-based processes have been the subject of research for the last few years. The aim of this study was the application of two biocatalysts, isolated lipase CALB and carrot root, for hydrolysis (R, S)-1-phenylethyl acetate within natural deep eutectic solvents. Based on the observed reaction yields, enantiomeric excess, specific enzymatic productivity and enzyme volumetric productivity, it can be concluded that natural deep eutectic solvents were superior to hydrolysis of (R, S)-1-phenylethyl acetate with isolated lipase according to volatile organic solvents. Hydrolysis by carrot had no significant impact on yield and productivity compared to organic solvents, but there was a clear improvement in enantioselectivity. The presented results contribute to the development of an efficient, economical and sustainable processes for the hydrolysis of industrially important compounds.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:381555
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2017-07-20 10:55:57

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