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master's thesis
Pojačana ekspresija odabranih gena i rezistencija kvasca Saccharomyces cerevisiae na inhibitore rasta
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:414100
Zandona, Antonio
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

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Zandona, A. (2016). Pojačana ekspresija odabranih gena i rezistencija kvasca Saccharomyces cerevisiae na inhibitore rasta (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:414100

Zandona, Antonio. "Pojačana ekspresija odabranih gena i rezistencija kvasca Saccharomyces cerevisiae na inhibitore rasta." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:414100

Zandona, Antonio. "Pojačana ekspresija odabranih gena i rezistencija kvasca Saccharomyces cerevisiae na inhibitore rasta." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:414100

Zandona, A. (2016). 'Pojačana ekspresija odabranih gena i rezistencija kvasca Saccharomyces cerevisiae na inhibitore rasta', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:414100

Zandona A. Pojačana ekspresija odabranih gena i rezistencija kvasca Saccharomyces cerevisiae na inhibitore rasta [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 April 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:414100

A. Zandona, "Pojačana ekspresija odabranih gena i rezistencija kvasca Saccharomyces cerevisiae na inhibitore rasta", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:414100

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Metadata
TitlePojačana ekspresija odabranih gena i rezistencija kvasca Saccharomyces cerevisiae na inhibitore rasta
Title (english)Overexpression of selected genes and resistance of yeast Saccharomyces cerevisiae on growth inhibitors
AuthorAntonio Zandona
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2016-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Proizvodnja bioetanola druge generacije temelji se na korištenju lignoceluloznih sirovina kao supstrata, no osnovni problem je nesposobnost proizvodnog mikroorganizma, kvasca Saccharomyces cerevisiae, da koristi ovakav supstrat za alkoholnu fermentaciju. Sirovinu je potrebno hidrolizirati, pri čemu nastaju spojevi koje kvasac može koristiti, ali i različiti spojevi koji djeluju kao inhibitori rasta i fermentacije (octena i levulinska kiselina, 2-furaldehid), zbog čega se nastoji konstruirati proizvodne sojeve koji bi bili rezistentni na inhibitore. Cilj istraživanja bio je utvrditi da li pojačana ekspresija kvaščevih gena ATR1, FLR1, YAP1, GSH1, CTA1 i ZWF1 povećava rezistenciju odabranih sojeva na inhibitore rasta. U ovome radu korišteni su kontrolni homozigotni i hibridni heterozigotni diploidni sojevi kvasca transformirani plazmidima koji sadrže pojedinačne navedene gene. Utvrđeno je da pojačana ekspresija gena ATR1 i YAP1 rezultira povećanom rezistencijom sojeva na inhibitore rasta (octenu i levulinsku kiselinu te 2-furaldehid) u odnosu na ishodne sojeve.
Abstract (english)
Second generation bioethanol production is based on the use of lignocellulosic composites as substrates, but the main problem is that the producer microorganism, the yeast S. cerevisiae, cannot use this substrate for fermentation. Therefore composites are hydrolysed to produce compounds that can be used by the yeast, but during hydrolysis different compounds that inhibit yeast growth are produced. Therefore, the aim is to construct producer strains that will be resistant to different growth inhibitors (acetic and levulinic acid and 2-furaldehyde). The aim of this research was to determine if the overexpression of yeast genes ATR1, FLR1, YAP1, GSH1, CTA1 or ZWF1 can increase the resistance of producer strains to inhibitors. In this research, homozygous control and heterozygous diploid strains were transformed with plasmids that allow overexpression of selected genes. It was determined that the overexpression of ATR1 and YAP1 genes in different strains results in the increased resistance to growth inhibitors (acetic and levulinic acid and 2-furaldehyde) compared to starting ones.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:414100
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2017-04-25 10:58:46

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