prikaz prve stranice dokumenta In vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela
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undergraduate thesis
In vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. urn:nbn:hr:159:169619
Pranklin, Andrea
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Cite this document

Pranklin, A. (2013). In vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:169619

Pranklin, Andrea. "In vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:169619

Pranklin, Andrea. "In vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:169619

Pranklin, A. (2013). 'In vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:169619

Pranklin A. In vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2013 [cited 2024 April 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:169619

A. Pranklin, "In vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:159:169619

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Metadata
TitleIn vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela
Title (english)In vitro adhesion of the S-layer expressing Lactobacillus strains to the proteins of the extracellular matrix
AuthorAndrea Pranklin
MentorJasna Novak (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2013-07-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada je ispitati in vitro adheziju pojedinih Lactobacillus sojeva, koji eksprimiraju S-layer površinski protein, na proteine ekstracelularnog matriksa. Eksperimenti su provedeni s četiri različita Lactobacillus soja: Lactobacillus brevis ZG1-K7, Lactobacillus brevis SF9B, Lactobacillus paraplantarum SF15B i Lactobacillus plantarum D6, iz Zbirke bakterija mliječne kiseline Laboratorija za tehnologiju antibiotika, enzima, probiotika i starter kultura, Prehrambeno-biotehnološkog fakulteta, koja sadrži više od 200 različitih sojeva bakterija mliječne kiseline. SDS-PAGE analizom površinskih proteina ovih Lactobacillus sojeva ustanovljeno je da eksprimiraju S-layer površinske proteine. Sposobnost adhezije Lactobacillus sojeva se međusobno razlikuje ovisno o soju i specifična je ovisno o proteinu ekstracelularnog matriksa. Uklanjanje intaktnog S-layer proteina s površine bakterijske stanice, ekstrakcijom s gvanidin hidrokloridom, uzrokovalo je smanjenje adhezije kod svih Lactobacillus sojeva na fibronektin i kod većine sojeva na kolagen i laminin, dok je adhezija na laminin bila povećana, ali neznatno kod sojeva L. brevis ZG1-K7 i L. paraplantarum SF15B, te na kolagen kod sojeva L. brevis SF9B i L. paraplantarum SF15B. Prema rezultatima odabrani Lactobacillus sojevi se uspješno adheziraju na proteine ECM, što se značajno smanji nakon tretmana bakterijskih stanica s proteinazom K.
Abstract (english)
The aim of this work was to characterize ability of several S-layer-expressing Lactobacillus to adhere to the main proteins of the extracellular matrix (ECM). Four different Lactobacillus strains from the Collection of Lactic Acid Bacteria (ZBMK in Laboratory of Antibiotic, Enzyme, Probiotic and Starter Cultures Technology, Faculty of Food Technology and Biotechnology, over 200 strains), were used: Lactobacillus brevis ZG1-K7, Lactobacillus brevis SF9B; Lactobacillus paraplantarum SF15B and Lactobacillus plantarum D6. Four Lactobacillus pure cultures exhibited S-layer proteins according to the SDS-PAGE analyses. The binding capability of the Lactobacillus strains varied greatly among the isolates and was specific depending of ECM protein. Removal of the intact S-layer proteins from the bacterial surface by extraction with guanidine hydrochloride has reduced the adhesion of all of Lactobacillus strains to fibronectin and in most strains to laminin and collagen; whereas, the adhesiveness to laminin was increased, althought slightly, with L. brevis ZG1-K7 and L. paraplantarum SF15B, and to collagen with L. brevis SF9B and L. paraplantarum SF15B. Binding tests showed that mostly selected Lactobacillus strains adhere to ECM proteins in a proteinase K-sensitive manner.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:169619
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2017-05-03 07:57:26

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