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master's thesis
Bioinformatička analiza alkan 1-monooksigenaza otkrivenih u metagenomu sedimenta sjevernog Jadrana
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:817190
Kolesarić, Domagoj
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Section for Bioinformatics

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Kolesarić, D. (2015). Bioinformatička analiza alkan 1-monooksigenaza otkrivenih u metagenomu sedimenta sjevernog Jadrana (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:817190

Kolesarić, Domagoj. "Bioinformatička analiza alkan 1-monooksigenaza otkrivenih u metagenomu sedimenta sjevernog Jadrana." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:817190

Kolesarić, Domagoj. "Bioinformatička analiza alkan 1-monooksigenaza otkrivenih u metagenomu sedimenta sjevernog Jadrana." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:817190

Kolesarić, D. (2015). 'Bioinformatička analiza alkan 1-monooksigenaza otkrivenih u metagenomu sedimenta sjevernog Jadrana', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 April 2024, https://urn.nsk.hr/urn:nbn:hr:159:817190

Kolesarić D. Bioinformatička analiza alkan 1-monooksigenaza otkrivenih u metagenomu sedimenta sjevernog Jadrana [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 April 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:817190

D. Kolesarić, "Bioinformatička analiza alkan 1-monooksigenaza otkrivenih u metagenomu sedimenta sjevernog Jadrana", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:817190

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Metadata
TitleBioinformatička analiza alkan 1-monooksigenaza otkrivenih u metagenomu sedimenta sjevernog Jadrana
Title (english)Bioinformatics analysis of alkane 1-monooxygenases discovered in metagenomic samples of sediment from the Adriatic sea
AuthorDomagoj Kolesarić
MentorJurica Žučko (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Section for Bioinformatics)
Zagreb
Defense date and country2015-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Istraživanje je provedeno s ciljem funkcionalne karakterizacije dviju vjerojatnih AlkB monooksigenaza otkrivenih u metagenomskom uzorku morskog sedimenta prikupljenog iz pristaništa za tankere u Puli gdje postoji selektivni pritisak alkana iz nafte na mikroorganizme tog staništa. Filogenetska analiza vjerojatnih AlkB monooksigenaza je provedena u programu MEGA 6 kojom se utvrdio evolucijski međuodnos potencijalnih AlkB monooksigenaza iz metagenomskog uzorka i već opisanih AlkB enzima dostupnih u bazama podataka. Funkcionalna karakterizacija je provedena pomoću resursa: CDD, InterPro i UniProt. Funkcionalnom analizom je potvrđeno pripadanje enzima alkan 1-monooksigenazama i određena su aktivna mjesta. Strukturno modeliranje je izvršeno pomoću SWISS-MODEL i I-TASSER web servisa za 3D modeliranje. Neuspješno 3D modeliranje pripisuje se nedostatku homolognih proteina kojima je eksperimentalno određena struktura.
Abstract (english)
This study has been aimed at the functional characterization of two putative alkane 1-monooxygenase discovered in a metagenomic sample of sea sediment collected in the docking bay in Pula where alkanes from oil are present in a higher concentration than in other environments. The phylogenetic analysis of putative alkane 1-monooxygenases has been conducted using MEGA 6 with the goal of determining the evolutionary connection between the putative alkane 1-monooxygenases and the well known AlkB enzymes. The web services CDD, InterPro and UniProt have been used for the functional characterization of the enzymes which determined similarities between the putative proteins and alkane 1-monooxygenases. For the structural modeling the web services SWISS-MODEL and I-TASSER have been used but with little success. This is explained by the lack of homologous proteins with experimentally determined structures.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:817190
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2017-05-05 09:46:27

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