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master's thesis
Razvoj HPLC-DAD metode za istovremenu analizu odabranih analgoantipiretika u kombiniranim ljekovitim oblicima
Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. urn:nbn:hr:163:915858
Rački, Bruno
University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical analysis

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Rački, B. (2018). Razvoj HPLC-DAD metode za istovremenu analizu odabranih analgoantipiretika u kombiniranim ljekovitim oblicima (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:915858

Rački, Bruno. "Razvoj HPLC-DAD metode za istovremenu analizu odabranih analgoantipiretika u kombiniranim ljekovitim oblicima." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:163:915858

Rački, Bruno. "Razvoj HPLC-DAD metode za istovremenu analizu odabranih analgoantipiretika u kombiniranim ljekovitim oblicima." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:163:915858

Rački, B. (2018). 'Razvoj HPLC-DAD metode za istovremenu analizu odabranih analgoantipiretika u kombiniranim ljekovitim oblicima', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:163:915858

Rački B. Razvoj HPLC-DAD metode za istovremenu analizu odabranih analgoantipiretika u kombiniranim ljekovitim oblicima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2018 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:163:915858

B. Rački, "Razvoj HPLC-DAD metode za istovremenu analizu odabranih analgoantipiretika u kombiniranim ljekovitim oblicima", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:163:915858

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Metadata
TitleRazvoj HPLC-DAD metode za istovremenu analizu odabranih analgoantipiretika u kombiniranim ljekovitim oblicima
Title (english)Development of HPLC-DAD method for concominant analisys of selected analgoantipyretics in combined medicinal products
AuthorBruno Rački
MentorMiranda Sertić (mentor)
Committee memberMiranda Sertić (predsjednik povjerenstva)
Committee memberJasmina Lovrić (član povjerenstva)
Committee memberŽivka Juričić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of pharmaceutical analysis)
Zagreb
Defense date and country2018-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Antipiretici i analgetici jedni su od najprodavanijih i najkorištenijih OTC lijekova u ljekarnama. Koriste se za liječenje tegoba glavobolja, zubobolja, bolova lokomotornog sustava i snižavanje povišene temperature. Postoje različite kombinacije antipiretika i analgetika uz dodatak tvari kao što su kofein i askorbinska kiselina. Cilj rada bio je razviti HPLC-DAD metodu za istovremenu analizu paracetamola, acetilsalicilne kiseline i kofeina kao farmaceutskih sastojaka koje dolaze u kombiniranim ljekovitim oblicima dostupnim na tržištu. Za analizu je korištena Symmetry (Waters) C18 kolona, 150 x 4,6 mm, 3,5 µm. Tijekom razvoja metode ispitani su različiti sastavi mobilne faze pri različitim brzinama protoka (0,8 – 1,5 mL/min) i različitim temperaturama analize (25 – 40 °C). Optimalnom se pokazala izokratna eluacija uz mobilnu fazu u sastavu acetonitril:H2O:HCOOH = 15:85:0,1, v/v pri brzini protoka 1,5 mL/min i temperaturi od 40 °C na valnoj duljini 237 nm. Ova jednostavna i brza metoda (10 min) je uspješno primijenjena za istovremenu analizu sva tri sastojka u gotovom ljekovitom obliku.
Abstract (english)
Antipyretics and analgesics are one of the best-selling OTC products found in pharmacies. They are used to treat headache, toothache, pains in locomotory system and to lower elevated body temperature. There are many products containing antipyretics and analgesics with substances like caffeine and ascorbic acid. The aim of this thesis was to develop HPLC-DAD method for simultaneous analysis of paracetamol, acetylsalicylic acid and caffeine as pharmaceutical ingredients of combined products available on the market. Symmetry (Waters) C18 column, 150 x 4.6 mm, 3.5 µm was used for the analysis. During method development, several different mobile phases were tested, at different flow rates (0.8 – 1.5 mL/min) and different column temperatures (25 – 40 °C). The optimal conditions were the mobile phase containing acetonitrile:water:formic acid = 15:85:0.1, v/v, isocratic elution, flow rate of 1,5 mL/min and the column temperature of 40 °C. Detection was performed at 237 nm wavelength. This simple and fast method (10 min) was successfully applied for the simultaneous analysis of all three pharmaceutic ingredients in combined drug product.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:163:915858
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on2020-02-21 14:20:47

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