In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita

Katanec, Antonija

prikaz prve stranice dokumenta In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita

Download
PDF 1.23 MB

master's thesis

In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. urn:nbn:hr:163:440457

Katanec, Antonija

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical technology

Cite this document

APA 6th Edition

Katanec, A. (2019). In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:440457

MLA 8th Edition

Katanec, Antonija. "In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:163:440457

Chicago 17th Edition

Harvard

Katanec, A. (2019). 'In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 25 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:440457

Vancouver

Katanec A. In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2019 [cited 2025 January 25] Available at: https://urn.nsk.hr/urn:nbn:hr:163:440457

IEEE

A. Katanec, "In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:163:440457

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:440457

Please login to the repository to save this object to your list.

Metadata

Title	In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita
Title (english)	In vitro biocompatibility study of liposomes differing in (phospho)lipid composition with the keratinocyte cells
Author	Antonija Katanec
Mentor	Željka Vanić (mentor)
Committee member	Željka Vanić (predsjednik povjerenstva)
Committee member	Anita Hafner (član povjerenstva)
Committee member	Dubravka Vitali Čepo (član povjerenstva)
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of pharmaceutical technology) Zagreb
Defense date and country	2019-07-11, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Pharmacy
Abstract	Različite vrste liposoma, konvencionalni, deformabilni, propilenglikol i kationski liposomi s uklopljenim azitromicinom, pripremljeni su film metodom. Ekstruzijom kroz polikarbonske membrane, formulacije liposoma su homogenizirane (indeks polidisperznosti < 0,43), a srednji promjeri značajno smanjeni (< 250 nm). Fosfolipidni sastav liposoma imao je utjecaja na površinski naboj liposoma. Konvencionalni, deformabilni i propilenglikol liposomi imali su zeta potencijale od -48 do -44 mV, dok je zeta potencijal kationskih liposoma iznosio + 62 mV. In vitro ispitivanja biokompatibilnosti liposoma s uklopljenim azitromicinom provedena na HaCaT stanicama procijenjena su pomoću MTT testa, mjerenjem metaboličke aktivnosti keratinocita 24 sata nakon inkubacije liposoma s HaCaT stanicama. Uklapanjem u liposome citotoksičnost azitromicina je smanjena. Fosfolipidni sastav i prisustvo suotapala u liposomima su imali utjecaja na biokompatibilnost. Propilenglikol je pokazao povoljan učinak na stanice keratinocita, dok su natrijev deoksikolat i DODAB pokazali potencijalnu citotoksičnost, ali tek pri izuzetno visokim koncentracijama lipida. Imajući u vidu koncentracije azitromicina u liposomima s kojima je postignut odgovarajući antibakterijski učinak, svi ispitivani tipovi liposoma su bili biokompatibilni s HaCaT stanicama.
Abstract (english)	Conventional, deformable, propylene glycol and cationic liposomes encapsulating azithromycin were prepared by the film hydration method. Extrusion of the liposomal dispersions through the polycarbonate membranes resulted with liposomes of more homogenous size distributions with a polydispersity indexes < 0,43 nm and a significantly reduced mean diameters (< 250 nm). The (phospo)lipid composition affected the surface charge of the liposomes. Zeta potential values for the conventional, deformable and propylene glycol liposomes ranged from -48 mV to -44 mV, while the cationic liposomes were shown to have a positive zeta potential of +62 mV. In vitro biocompatibilities of the different types of liposomes containing azithromycin with the human HaCaT cells were evaluated 24 h after the incubation of the cells with the liposomes using the cellular metabolic activity (MTT assay). The encapsulation of azithromycin in liposomes reduced the cytotoxicity of the drug, whereas the (phospo)lipid composition and the presence of the surfactant and cosolvent affected the biocompatibility. Propylene glycol was well tolerated by the keratinocytes in comparison to sodium deoxycholate and DODAB, where potential cytotoxicities were observed. All types of the liposomes were biocompatible with the keratinocytes even at the very high concentrations of azithromycin tested.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:440457
Study programme	Title: Pharmacy Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2020-02-21 16:23:59