Conventional, deformable and propylene glycol liposomes encapsulating azythromicin were prepared by the film hydration method. In order to obtain homogenous liposomal dispersions and optimize vesicle size for dermal application, liposomal preparations were extruded through polycarbonate membranes with pore size of 400 nm and 100 nm. Resulting vesicles were in the mean diameter range of 113 to 140 nm, with low values of polydispersity index measured (0,100 – 0,138). Negative values of zeta potential for all liposomal preparations (between -36 mV and -45 mV) indicated the formation of physically stable liposomal dispersions. In vitro release studies were performed during 24 h using Franz diffusion cell system. The release of azythromicin from different liposomes and control (azithromycin in 40% ethanol solution) was tested. The amount of the released azithromycin was determined cromatographically (HPLC). All of the liposomal formulations were characterized by the slower release rates and sustained release profiles as compared to the control (solution of the drug). Among the investigated liposomes, the release rates were higher for elastic vesicles (deformable and propylene glycol liposomes) than conventional liposome and the highest release rate was observed for deformable liposomes. It could be concluded that the azithromycin release from the phospholipid vesicles depends on their physicochemical properties and the presence of the penetration enhancer or edge activator in their composition. Elastic liposomes have been shown to be suitable as azithromycin carrier systems for dermal application which could possibly achieve prolonged and controlled release of the drug.