Protein glycosylation is the most diverse posttranslational modification of proteins, with N-glycosylation being the most common one and the most extensively studied. The correlation between the N-glycan profile of plasma proteins and numerous pathological conditions is well known, particularly for those conditions with a significant inflammatory component. This notion made the analysis of the glycan profile of plasma proteins promising. Understanding the patient’s glycan profile and the ability to analyse it with high-throughput methods would contribute to the personalization of medical intervention considerably. HILIC-UPLC, besides being high-throughput, is a very sensitive analytical method allowing the replacement of impractical and unpleasant venepuncture sampling with a sampling of capillary blood on a filter paper. Along with the filter paper, numerous sophisticated methods for microsampling of capillary blood have recently been developed. In this research filter paper (Whatman), Mitra sticks (Neoteryx) and plasma separator (Noviplex) devices for capillary blood microsampling were evaluated for the purpose of the plasma protein glycan profile analysis. The comparability of the mentioned microsampling methods with venepuncture were tested in the first phase of the research, whereas the repeatability of microsampling methods were assessed in the second phase. In the first phase samples from ten healthy donors were acquired. One sample was collected for each microsampling method and venepuncture. In the second phase, the samples from five donors were collected in sextuplicate for each microsampling method. The analysis of the N-glycan profile of plasma proteins was done by the HILIC-UPLC. The collected data was interpreted by descriptive statistics. Results of the first phase, i.e., comparability, showed the surpassing equivalence of the samples acquired by the plasma separator and a very good equivalence of the samples acquired by Mitra sticks to the standard venepuncture sampling. These microsampling methods have proved superior to the sampling on filter paper. Results of the second phase, i.e., repeatability, attested the excellent repeatability of the plasma separator method and the lowest for sampling on the filter paper. The studied performances of microsampling methods have clearly shown the analytical superiority of the plasma separator and Mitra stick sampling methods compared to the filter paper. However, other method properties, e.g., price of sampling devices, ease of sampling, degree of correctly sampled blood, and the ability of analysis automatization also take part in the selection of the sampling method for N-glycan profile analysis.