Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin

Veltruski, Sanja

prikaz prve stranice dokumenta Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin

master's thesis

Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. urn:nbn:hr:163:145645

Veltruski, Sanja

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

Cite this document

APA 6th Edition

Veltruski, S. (2021). Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:145645

MLA 8th Edition

Veltruski, Sanja. "Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:145645

Chicago 17th Edition

Harvard

Veltruski, S. (2021). 'Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 18 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:145645

Vancouver

Veltruski S. Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2021 [cited 2025 January 18] Available at: https://urn.nsk.hr/urn:nbn:hr:163:145645

IEEE

S. Veltruski, "Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:163:145645

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:145645

Please login to the repository to save this object to your list.

Metadata

Title	Ispitivanje stabilnosti ekstrakta fekalnog kalprotektina upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash Calprotectin
Title (english)	Stability testing of fecal calprotectin extract using QUANTA Flash Calprotectin commercial extraction system
Author	Sanja Veltruski
Mentor	Donatella Verbanac (mentor)
Committee member	Donatella Verbanac (predsjednik povjerenstva)
Committee member	Roberta Petlevski (član povjerenstva)
Committee member	Andrea Tešija Kuna (član povjerenstva)
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of medical biochemistry and haematology) Zagreb
Defense date and country	2021-11-03, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Medical Biochemistry
Abstract	Fekalni kalprotektin je važan biomarker za razlikovanje upalnih crijevnih bolesti (IBD, engl. inflamatory bowel disease) od sindroma iritabilnog crijeva (IBS, engl. irritable bowel syndrome), kao i za praćenje tijeka bolesti i recidiva. Metoda je neinvazivna, a pacijent se upućuje na kolonoskopiju u slučaju povišene koncentracije fekalnog kalprotektina odnosno, ukoliko se sumnja na IBD. U ovom radu ispitana je stabilnost 20 uzoraka tijekom 120 sati. Koncentracija fekalnog kalprotektina se mjerila kemiluminiscentnom imunoanalizom (CLIA) upotrebom komercijalnog sustava za ekstrakciju QUANTA Flash® Calprotectin (INOVA Diagnostics, San Diego, CA, USA) na analizatoru BIO-FLASH (INOVA Diagnostics, San Diego, CA, USA). Zaključno, 85% ekstrakata koji su priređeni i obrađeni po uputama proizvođača zadovoljili su traženim uvjetima stabilnosti.
Abstract (english)	Fecal calprotectin is an essential biomarker for distinguishing inflammatory bowel disease (IBD) from irritable bowel syndrome (IBS) and monitoring the course of disease and relapse. The method is non-invasive, and the patient is referred for colonoscopy in case of elevated fecal calprotectin concentration, particularly if IBD is suspected. In this experiment, the stability of 20 samples over 120 hours was examined. Fecal calprotectin concentration was determined by chemiluminescent immunoassay (CLIA) method using a commercial QUANTA Flash® Calprotectin extraction system (INOVA Diagnostics, San Diego, CA, USA) on a BIO-FLASH analyzer (INOVA Diagnostics, San Diego, CA, USA). Finally, 85% of the extracts prepared and processed according to the manufacturer's instructions meet the required stability requirements.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:145645
Study programme	Title: Medical biochemistry Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra medicinske biokemije i laboratorijske medicine (magistar/magistra medicinske biokemije i laboratorijske medicine)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2022-02-09 10:39:01