Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina

Tomašić, Antonela

prikaz prve stranice dokumenta Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina

Download
PDF 723.44 KB

master's thesis

Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. urn:nbn:hr:163:103179

Tomašić, Antonela

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of general and inorganic chemistry

Cite this document

APA 6th Edition

Tomašić, A. (2021). Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:103179

MLA 8th Edition

Tomašić, Antonela. "Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:103179

Chicago 17th Edition

Tomašić, Antonela. "Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:103179

Harvard

Tomašić, A. (2021). 'Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 23 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:103179

Vancouver

Tomašić A. Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2021 [cited 2025 January 23] Available at: https://urn.nsk.hr/urn:nbn:hr:163:103179

IEEE

A. Tomašić, "Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:163:103179

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:103179

Please login to the repository to save this object to your list.

Metadata

Title	Iskorištenje metode kromatofokusiranja za razdvajanje sijaloformi humanog serumskog transferina
Title (english)	Recovery of chromatofocusing for separation of human serum transferrin sialophorms
Author	Antonela Tomašić
Mentor	Mario Gabričević (mentor)
Committee member	Mario Gabričević (predsjednik povjerenstva)
Committee member	Ana Budimir (član povjerenstva)
Committee member	Miranda Sertić (član povjerenstva)
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of general and inorganic chemistry) Zagreb
Defense date and country	2021-07-16, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Pharmacy
Abstract	Promjene u glikozilaciji, odnosno sijalilaciji humanog serumskog transferina događaju se kod brojnih akutnih i kroničnih bolesti te mogu biti jedan od pokazatelja stanja oboljenja. Razdvajanje različitih sijaloformi transferina korisno je za brojna znanstvena istraživanja i u dijagnostičke svrhe, ali često zahtijeva korištenje skupih analitičkih metoda. Ideja ovog rada bila je testirati kromatofokusiranje kao jeftiniju i jednostavniju metodu razdvajanja sijaloformi transferina za daljnje analize, uz naglasak na iskorištenje metode. Kromatofokusiranje s linearnim pH gradijentom pokazalo se kao metoda koja ima velik potencijal postati učinkovita i praktična metoda za analizu transferina i sličnih proteina, koja ima zadovoljavajuće iskorištenje i razdvajanje sijaloformi transferina.
Abstract (english)	Changes in glycosylation or sialylation of human serum transferrin occur in a number of acute and chronic diseases. Separation of various transferrin sialoforms is useful for numerous scientific studies and for diagnostic purposes but often requires the use of expensive analytical methods. The goal of this study was to test chromatofocusing as a cheaper and simpler method of separating transferrin sialoforms for further analysis, with emphasis on recovery. Chromatofocusing with a linear pH gradient has proven to be a method that has great potential in becoming an efficient and practical method for the analysis of transferrin and other glycoproteins, with sufficient recovery and satisfactory separation of protein sialoforms.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:103179
Study programme	Title: Pharmacy Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access Embargo expiration date: 2023-07-16
Terms of use
Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2022-02-28 13:08:39