Greater or broadleaf plantain (Plantago major L., Plantaginaceae) is a widespread plant, known in folk medicine for many years. It contains numerous biologically active compounds including flavonoids, alkaloids, terpenoids, iridoids, vitamins, polysaccharides, fatty and phenolic acids. Due to its chemical constituents, it has numerous biological effects. For example, it promotes wound healing, prevents viral and bacterial infections, it is useful in treatment of diarrhea, and lowers the level of glucose and cholesterol in the blood. Polyphenols act as antioxidants, anti-inflammatory and reduce the risk of some chronic diseases, eg cardiovascular. In this work, a fast and efficient extraction method, ultrasonic extraction, was used for the extraction of polyphenols from P. major. The amount of total polyphenols, flavonoids and phenolic acids was determined spectrophotometrically. Verbascoside concentration was determined using HPLC. By comparing the concentrations of the target compounds and the conditions in which extracts were prepared, the parameters necesary for the most efficient extraction of individual polyphenols were established. The extract with the highest content of total polyphenols was prepared using 70 % GL, 5 % lactic acid and was extracted for 30 min at a temperature of 70 °C with an ultrasound power of 144 W. Flavonoids were best extracted using 70 % GL, without lactic acid, and the extraction lasted 30 min at 70 °C with an ultrasound power of 720 W. The most suitable medium for phenolic acid was with 10 % GL, 0.3 mmol HP-β- CD, and the extraction lasted 30 min at 20 °C with an ultrasound power of 144 W. The extract with 10 % GL, 5% lactic acid, 0.3 mmol HP-β-CD showed the highest verbascoside extraction, and the extraction time was 10 min at 20 °C and with an ultrasound power of 144 W. It is still necessary to statistically process the results in order to precisely determine how certain parameters affect the extraction of all the mentioned polyphenol components.