Procjena morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60

Vučetić, Josipa

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master's thesis

Procjena morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2023. urn:nbn:hr:163:207457

Vučetić, Josipa

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

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APA 6th Edition

Vučetić, J. (2023). Procjena morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60 (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:207457

MLA 8th Edition

Vučetić, Josipa. "Procjena morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2023. https://urn.nsk.hr/urn:nbn:hr:163:207457

Chicago 17th Edition

Harvard

Vučetić, J. (2023). 'Procjena morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 19 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:207457

Vancouver

Vučetić J. Procjena morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2023 [cited 2025 January 19] Available at: https://urn.nsk.hr/urn:nbn:hr:163:207457

IEEE

J. Vučetić, "Procjena morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:163:207457

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:207457

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Title	Procjena morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60
Title (english)	Evaluation of erythrocytes morphological characteristics using Sysmex DI-60 digital morphology analyzer
Author	Josipa Vučetić
Mentor	Dunja Rogić (mentor)
Committee member	Dunja Rogić (predsjednik povjerenstva)
Committee member	Ana Kozmar (član povjerenstva)
Committee member	Lara Milevoj Kopčinović (član povjerenstva)
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of medical biochemistry and haematology) Zagreb
Defense date and country	2023-07-25, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Medical Biochemistry
Abstract	Poremećaji eritrocita se odražavaju karakterističnim morfološkim promjenama, stoga je bitno detektirati i kvantificirati morfološke abnormalnosti. Hematološki analizatori daju pouzdane rezultate kompletne krvne slike u većini slučajeva, ali u prisutnosti morfoloških promjena eritrocita još uvijek ne mogu ponuditi cjelovitu kliničku sliku. U tu svrhu izvodi se pregled razmaza periferne krvi koji se posljednjih godina nastoji automatizirati, a razvojem umjetne inteligencije i algoritama za digitalnu mikroskopiju to postaje moguće. Cilj ovog rada bio je ispitati pouzdanost automatizirane procjene morfoloških karakteristika eritrocita digitalnom mikroskopijom na uređaju Sysmex DI-60, kao dio verifikacije novog uređaja za digitalnu morfologiju u svrhu uvođenja automatizirane procjene morfoloških karakteristika eritrocita u rutinski laboratorijski rad. Ispitana je korelacija šest morfoloških karakteristika (polikromazija, hipokromija, anizocitoza, mikrocitoza, makrocitoza, poikilocitoza) dobivenih na uređaju za digitalnu mikroskopiju, s parametrima dobivenima na hematološkom analizatoru Sysmex XN-3100 (Rtc, MCH, RDW, MCV). Utvrđena je umjerena korelacija između hipokromije i MCH, mikrocitoze i MCV, anizocitoze i RDW. Slaba korelacija utvrđena je između polikromazije i broja retikulocita te između makrocitoze i MCV. Kako bi slaganje između ovih parametara bilo bolje, potrebno je poboljšati same algoritme digitalne mikroskopije, ali i prilagoditi kriterije za stupnjevanje pojedinih karakteristika.
Abstract (english)	Erythrocytes' disorders are reflected in characteristic morphological changes, therefore it is essential to detect and quantify them. Hematology analyzers provide reliable results of a complete blood count in most cases, but in the presence of erythrocytes' morphological changes they still cannot offer a complete clinical picture. In this case, inspection of the peripheral blood smear is performed. In recent years, there have been attempts made to automate this process, and with the development of artificial intelligence and algorithms for digital microscopy, this became possible. The aim of this thesis was to test the reliability of the automated assessment of erythrocytes' morphological characteristics using Sysmex DI-60 digital morphology analyzer. The correlation of six morphological characteristics (polychromasia, hypochromia, anisocytosis, microcytosis, macrocytosis, poikilocytosis) obtained on a Sysmex DI-60 with parameters obtained on a hematology analyzer Sysmex XN-3100 (Rtc, MCH, RDW, MCV) was examined. Moderate correlation was found between hypochromia and MCH, microcytosis and MCV, anisocytosis and RDW. Weak correlation was found between polychromasia and reticulocytes' count as well as between macrocytosis and MCV. In order to obtain better agreement between these parameters, it is necessary to improve the digital microscopy algorithm, but also to adjust the criteria for grading individual characteristics.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:207457
Study programme	Title: Medical biochemistry Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2023-12-07 13:14:56