Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova

Jurina, Ena

prikaz prve stranice dokumenta Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova

Embargo period 2025-06-13

master's thesis

Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2023. urn:nbn:hr:163:342451

Jurina, Ena

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical technology

Cite this document

APA 6th Edition

Jurina, E. (2023). Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:342451

MLA 8th Edition

Jurina, Ena. "Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2023. https://urn.nsk.hr/urn:nbn:hr:163:342451

Chicago 17th Edition

Harvard

Jurina, E. (2023). 'Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 16 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:342451

Vancouver

Jurina E. Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2023 [cited 2025 January 16] Available at: https://urn.nsk.hr/urn:nbn:hr:163:342451

IEEE

E. Jurina, "Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:163:342451

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:342451

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Metadata

Title	Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova
Title (english)	Optimization of a procedure for performing in vitro release of ciprofloxacin from vesicular phospholipid gels
Author	Ena Jurina
Mentor	Željka Vanić (mentor)
Committee member	Željka Vanić (predsjednik povjerenstva)
Committee member	Zora Rukavina (član povjerenstva)
Committee member	Lovorka Vujić (član povjerenstva)
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of pharmaceutical technology) Zagreb
Defense date and country	2023-06-13, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Pharmacy
Abstract	Formulacije vezikularnih fosfolipidnih gelova (VFG), koje su se međusobno razlikovale fosfolipidnim sastavom, prisustvom polimera (kitozan) i suotapala (propilenglikol), pripremljene su hidratizacijom fosfolipida u vodenoj otopini ciprofloksacina uz primjenu visokotlačnog homogenizatora. Srednji promjer liposoma unutar VFG-a kretao se od 126 do 176 nm, uz indeks polidisperznosti manji od 0,43 te zeta potencijal od +25 do +33 mV, dok je uspješnost uklapanja iznosila od 25 do 31%. Ispitivanje in vitro oslobađanja ciprofloksacina iz VFG-a provedena je primjenom metode membranske difuzije tijekom 30 sati. Ispitivanja su provedena u vodi te u puferu pH 7,4 kao medijima za oslobađanje. Kao kontrola služila je vodena otopina ciprofloksacina. Optimizacija postupka provedena je na formulaciji koja je sadržavala samo SPC 100, te su pod istim uvjetima provedena ispitivanja s formulacijama koje su uz SPC 100 sadržavale SLPC-80 i propilenglikol (elastični dvoslojevi), odnosno SPC 100 i kitozan. Oslobađanje je bilo najbrže iz formulacije karakterizirane elastičnim fosfolipidnim dvoslojevima, dok su druge dvije formulacije, kojima se fosfolipidni dvosloj sastojao od SPC 100, odnosno SPC 100 i kitozana, osiguravale sporije oslobađanje lijeka (neovisno o korištenom dijalizacijskom mediju).
Abstract (english)	Vesicular phospholipid gels (VPG), differing in phospholipid composition, presence of chitosan and/or propylene glycol, were prepared by hydration of phospholipids in an aqueous solution of ciprofloxacin, followed by homogenization in a high-pressure homogenizer. The mean diameters of liposomes inside the VPGs ranged from 126 to 176 nm, with a polydispersity indexes less than 0.43, and zeta potentials from +25 to +33 mV, while the encapsulation efficiency amounted from 25 to 31%. A membrane diffusion method was applied to test in vitro release of ciprofloxacin from the different VPGs in water and buffer, pH 7.4 during 30 hours. An aqueous solution of ciprofloxacin served as a control. Optimization of in vitro release conditions was conducted on VPG composed of only SPC 100. The optimized conditions were applied for performing in vitro release studies from VPGs which, in addition to SPC 100, contained SLPC 80 and propylene glycol (elastic bilayers), or SPC 100 and chitosan. Somewhat faster release of ciprofloxacin was achieved from the VPG characterized by elastic bilayers, while the other two formulations of VPGs exhibited slower drug release, regardless of the dialysis medium used.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:342451
Study programme	Title: Pharmacy Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resource	Text
File origin	Born digital
Access conditions	Embargoed access Embargo expiration date: 2025-06-13
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Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2024-01-04 15:56:49