Izolacija antioksidativnih sastavnica biljke Medicago sativa L. primjenom ciklodekstrina

Šimić, Sara

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master's thesis

Izolacija antioksidativnih sastavnica biljke Medicago sativa L. primjenom ciklodekstrina

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. urn:nbn:hr:163:522369

Šimić, Sara

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical technology

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APA 6th Edition

Šimić, S. (2015). Izolacija antioksidativnih sastavnica biljke Medicago sativa L. primjenom ciklodekstrina (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:522369

MLA 8th Edition

Šimić, Sara. "Izolacija antioksidativnih sastavnica biljke Medicago sativa L. primjenom ciklodekstrina." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2015. https://urn.nsk.hr/urn:nbn:hr:163:522369

Chicago 17th Edition

Harvard

Šimić, S. (2015). 'Izolacija antioksidativnih sastavnica biljke Medicago sativa L. primjenom ciklodekstrina', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 23 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:522369

Vancouver

Šimić S. Izolacija antioksidativnih sastavnica biljke Medicago sativa L. primjenom ciklodekstrina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2015 [cited 2025 January 23] Available at: https://urn.nsk.hr/urn:nbn:hr:163:522369

IEEE

S. Šimić, "Izolacija antioksidativnih sastavnica biljke Medicago sativa L. primjenom ciklodekstrina", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:163:522369

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:522369

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Title	Izolacija antioksidativnih sastavnica biljke Medicago sativa L. primjenom ciklodekstrina
Title (english)	Isolation of antioxidant components of Medicago sativa L. using cyclodextrins
Author	Sara Šimić
Mentor	Mario Jug (mentor)
Committee member	Mario Jug
Committee member	Jasna Jablan
Committee member	Željka Vanić
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of pharmaceutical technology) Zagreb
Defense date and country	2015-07-06, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Pharmacy
Abstract	Biljka lucerna (Medicago sativa L.) nalazi široku primjenu u ljekovite svrhe. Posjeduje antimikrobno, protuupalno, antikancerogeno i antioksidativno djelovanje zbog prisutnosti fenolnih komponenti koje se ponašaju kao slobodni radikali i kelatori metala. Za ekstrakciju fenolnih spojeva iz biljaka najčešće su korištena organska otapala koja negativno utječu na okoliš i ljude. Stoga je cilj ovog diplomskog rada bio optimizirati uvjete ekstrakcije fenolnih komponenti iz vrste Medicago sativa L. korištenjem vodene otopine hidroksipropil-β-ciklodekstrina. Korištenjem programa za statističko planiranje eksperimenta uspoređen je utjecaj različitih procesnih parametara na učinkovitost ekstrakcije. Određeni su najpovoljniji ekstrakcijski uvjeti u kojima je moguće ekstrahirati maksimalnu količinu polifenola, flavonoida i fenolnih kiselina te postići najbolju antioksidativnu aktivnost. Ispitan je i utjecaj liofilizacije na antioksidativnu aktivnost ekstrakata. Postupkom liofilizacije očuvana je količina fenolnih komponenti. Nakon procesa liofilizacije ekstrakata antiradikalna aktivnost raste, a kelirajuća blago pada, što znači da ukupan antioksidativni potencijal ekstrakata ostaje očuvan.
Abstract (english)	The Alfalfa (Medicago sativa L.) is widely used in medicinal purposes. The plant has been reported to have antimicrobial, anti-inflammatory, anticancer and antioxidant effects. These biological activities could be attributed to the presence of both phenolic compounds which act as free radical scavengers and metal chelators. Organic solvents are usually used for the extraction of phenolic compounds from plants which affect adversely on the environment and people. Therefore, the purpose of this diploma thesis was to optimize the extraction conditions of phenol components from Lotus corniculatus L. using aqueous solution of HPβCD. By using the programmes for the statistical design of the experiments it is compared the effect of different process parameters on the extraction efficiency. Some are more favorable extraction conditions in which it is possible to extract the maximum amount of polyphenols, flavonoids and phenolic acids and achieve the best antioxidant activity. The effect of lyophilization is also examined on the antioxidant activity of the extracts. The amount of phenolic components is preserved by freeze drying process. After lyophilization process extracts antiradical activity is growing, and chelating gently fall, which means that the total antioxidant potential of extracts remains preserved.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:522369
Study programme	Title: Pharmacy Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2016-01-18 16:36:23