Lakohlapljive polarne sastavnice maslinovog ulja kao biomarker kvalitete maslinovog ulja -primjena HSS-GC-FID tehnike

Bošnjak, Ines

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master's thesis

Lakohlapljive polarne sastavnice maslinovog ulja kao biomarker kvalitete maslinovog ulja -primjena HSS-GC-FID tehnike

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. urn:nbn:hr:163:355958

Bošnjak, Ines

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical analysis

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APA 6th Edition

Bošnjak, I. (2017). Lakohlapljive polarne sastavnice maslinovog ulja kao biomarker kvalitete maslinovog ulja -primjena HSS-GC-FID tehnike (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:355958

MLA 8th Edition

Bošnjak, Ines. "Lakohlapljive polarne sastavnice maslinovog ulja kao biomarker kvalitete maslinovog ulja -primjena HSS-GC-FID tehnike." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2017. https://urn.nsk.hr/urn:nbn:hr:163:355958

Chicago 17th Edition

Harvard

Bošnjak, I. (2017). 'Lakohlapljive polarne sastavnice maslinovog ulja kao biomarker kvalitete maslinovog ulja -primjena HSS-GC-FID tehnike', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 16 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:355958

Vancouver

Bošnjak I. Lakohlapljive polarne sastavnice maslinovog ulja kao biomarker kvalitete maslinovog ulja -primjena HSS-GC-FID tehnike [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2017 [cited 2025 January 16] Available at: https://urn.nsk.hr/urn:nbn:hr:163:355958

IEEE

I. Bošnjak, "Lakohlapljive polarne sastavnice maslinovog ulja kao biomarker kvalitete maslinovog ulja -primjena HSS-GC-FID tehnike", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:163:355958

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:355958

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Title	Lakohlapljive polarne sastavnice maslinovog ulja kao biomarker kvalitete maslinovog ulja -primjena HSS-GC-FID tehnike
Title (english)	Polar volatile compounds as olive oil quality biomarkers - application of HSS-GC-FID technique
Author	Ines Bošnjak
Mentor	Ana Mornar Turk (mentor)
Committee member	Ana Mornar Turk (predsjednik povjerenstva)
Committee member	Željan Maleš (član povjerenstva)
Committee member	Olga Gornik (član povjerenstva)
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of pharmaceutical analysis) Zagreb
Defense date and country	2017-08-29, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Pharmacy
Abstract	Uzgoj masline i proizvodnja maslinovog ulja je od davnina dio tradicije mediteranskog stanovništva pa tako i Republike Hrvatske. Maslinovo ulje se zahvaljujući prehrambenoj, terapeutskoj i preventivnoj ulozi sredinom dvadesetog stoljeća širi van granica Mediterana. Važnu ulogu u proizvodnji maslinovog ulja ima redovita kontrola kvalitete. Kvaliteta maslinovog ulja usko je povezana s prisutnošću lakohlapljivih polarnih sastvanica poput metanola i etanola budući da se smatra da ovi alkoholi nastaju tijekom propadanja ploda kao i tijekom skladištenja gotovog proizvoda. U ovom radu za analizu je korištena prethodno razvijena HSS-GC-FID metoda za određivanje prisutnosti i sadržaja lakohlapljivih polarnih sastavnica u maslinovu ulju. Prisutnost polarnih lakohlapljivih sastavnica u maslinovom ulju dokazana je usporedbom njihovih vremena zadržavanja na koloni u uzorku i u standardnim otopinama. Podaci o sadržaju etanola, metanola, 2-propanola, acetona i etil-acetata dobiveni su računski uz pomoć jednadžbi kalibracijskih krivulja te su bili manji od 5,1 ng/g uzorka. Da bi se dobili pouzdani rezultati mjerenja korišteni su kalibracijski postupci poput metode standardnog dodatka i metode unutarnjeg standarda. Rezultati istraživanja upućuju na mogućnost praćenja polarnih lakohlapljivih tvari kao biomarkera kvalitete maslinovog ulja, a korištena metoda HSS-GC-FID se pokazala kao vrlo učinkovita i pouzdana metoda za analizu lakohlapljivih sastavnica maslinovog ulja.
Abstract (english)	Olive tree cultivation and olive oil production has long been a part of the tradition of the Mediterranean population, including the Republic of Croatia. Olive oil, due to its nutritional, therapeutic and preventive role, expands a mid-twentieth century outside the borders of the Mediterranean. Regular quality control is important in olive oil production. The quality of olive oil is closely related to the presence of light volatile polar solvents such as methanol and ethanol as these alcohols are considered to occur during decaying of fruit as well as during storage of the finished product. In this research, a previously developed HSS-GC-FID method was used to determine the presence and content of volatile polar compounds in olive oil. The presence of polar volatile compounds in olive oil was demonstrated by comparing their retention times on columns in the oil sample and in the standard solutions. The contents of ethanol, methanol, 2-propanol, acetone and ethyl acetate were obtained by using the calibration equations and the results were less than 5.1 ng/g. In order to obtain reliable measurement results, calibration procedures such as the standard addition method and the internal standard method were used. The results of the research indicate the possibility of monitoring volatile polar compounds as a quality biomarkers of olive oil. Furthermore, the used method HSS-GC-FID has proven to be a highly efficient and reliable method for the analysis of volatile components of olive oil.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:355958
Study programme	Title: Pharmacy Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2017-11-24 16:36:12