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master's thesis
Ispitivanje fenotipa stanica humanog melanoma nakon tretmana vemurafenibom
Zagreb: University of Zagreb, Faculty of Science, 2019. urn:nbn:hr:217:639282
Bobić, Ivana
University of Zagreb
Faculty of Science
Department of Chemistry

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Bobić, I. (2019). Ispitivanje fenotipa stanica humanog melanoma nakon tretmana vemurafenibom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:639282

Bobić, Ivana. "Ispitivanje fenotipa stanica humanog melanoma nakon tretmana vemurafenibom." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:639282

Bobić, Ivana. "Ispitivanje fenotipa stanica humanog melanoma nakon tretmana vemurafenibom." Master's thesis, University of Zagreb, Faculty of Science, 2019. https://urn.nsk.hr/urn:nbn:hr:217:639282

Bobić, I. (2019). 'Ispitivanje fenotipa stanica humanog melanoma nakon tretmana vemurafenibom', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:639282

Bobić I. Ispitivanje fenotipa stanica humanog melanoma nakon tretmana vemurafenibom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2019 [cited 2024 April 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:639282

I. Bobić, "Ispitivanje fenotipa stanica humanog melanoma nakon tretmana vemurafenibom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:217:639282

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Metadata
TitleIspitivanje fenotipa stanica humanog melanoma nakon tretmana vemurafenibom
Title (english)Characterization of human melanoma cell phenotype after vemurafenib treatment
AuthorIvana Bobić
MentorMaja Herak Bosnar (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberSanda Rončević (član povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2019-02-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Maligni melanom je najagresivniji i najzloćudniji oblik raka kože. U većini melanoma utvrđeno je prisustvo mutacije gena BRAF pa liječenje uključuje i terapiju kemoterapeuticima, vrlo često vemurafenibom, inhibitorom enzima BRAF. Međutim, stanice melanoma obično razvijaju otpornost i dugotrajnim tretmanom vemurafenibom in vitro stanice s tipično epitelnom morfologijom poprimaju izduženi, vretenasti oblik. Takva promjena fenotipa upućuje na indukciju procesa epitelno-mezenhimalne tranzicije kojim stanice poprimaju još zloćudnija svojstva. Cilj ovog rada bio je okarakterizirati stanične linije humanog melanoma A375M i WM793B nakon višemjesečnog tretmana vemurafenibom utvrđivanjem vijabilnosti stanica, ekspresije i lokalizacije ključnih biljega epitelno-mezenhimalne tranzicije te ekspresije supresorā metastaziranja, NME1 i NME2. U tu svrhu korišteni su test MTT te metode Western blot i fluorescentna imunocitokemija.
Abstract (english)
Human melanoma is the most aggressive and most malignant of all skin cancers. Most cases of melanoma harbour BRAF gene mutation; therefore, treatment includes chemotherapeutics, commonly vemurafenib, a BRAF enzyme inhibitor. However, melanoma cells usually develop resistance and, after long-term vemurafenib treatment, cultured cells with an epithelial-like morphology acquire a more elongated, spindle-like shape. This change indicates the induction of a process called epithelial-mesenchymal transition which prompts even more malignant behaviour. The aim of this study was to characterize human melanoma cell lines A375M and WM793B after several months of vemurafenib treatment, by determining cell viability, expression and localization of key epithelial-mesenchymal transition markers and expression of metastasis suppressors, NME1 and NME2. For that purpose, MTT assay, Western blot and fluorescent immunocytochemistry methods were used.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:639282
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-02-15
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Science
Created on2019-02-18 13:21:25

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