Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače

Bošnjak, Marina

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master's thesis

Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače

Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:420966

Bošnjak, Marina

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Bošnjak, M. (2017). Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:420966

MLA 8th Edition

Bošnjak, Marina. "Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:420966

Chicago 17th Edition

Harvard

Bošnjak, M. (2017). 'Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače', Master's thesis, University of Zagreb, Faculty of Science, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:420966

Vancouver

Bošnjak M. Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:217:420966

IEEE

M. Bošnjak, "Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:420966

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:420966

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Title	Raznovrsnost alela lokusa STR_MICA među bolesnicima sa šećernom bolesti tipa 1 i autoimunom bolesti štitnjače
Title (english)	The diversity of STR_MICA alleles among patients with type 1 diabetes and autoimmune thyroid disease
Author	Marina Bošnjak
Mentor	Zorana Grubić (mentor)
Mentor	Ana Galov (mentor)
Committee member	Ana Galov (predsjednik povjerenstva)
Committee member	Jasna Lajtner (član povjerenstva)
Committee member	Zlatko Liber (član povjerenstva)
Committee member	Domagoj Đikić (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2017-02-27, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Cilj ovog rada bio je istražiti raspodjelu alela STR_MICA među bolesnicima sa šećernom bolesti tipa 1 (DMT1), autoimunim poliglandularnim sindromom tipa 3(APS3v) i autoimunom bolesti štitnjače (AITD) te utvrditi je li učestalost pojedinih alela STR_MICA ovisna o genima HLA-DRB1 ili je rezultat neravnoteže udruživanja između alela STR_MICA i gena HLA. U istraživanje je bilo uključeno 290 nesrodnih bolesnika (DMT1-86, APS3v-63 i AITD-141) koji su uspoređeni s kontrolnim ispitanicima (N=86) odgovarajućeg spola i dobi. Svim ispitanicima uzeto je 5 ml periferne krvi s EDTA iz koje je izolirana DNA. Uzorci su testirani za alele STR_MICA lančanom reakcijom polimeraze (PCR-STR) i potom analizirani elektroforezom na 6% poliakrilamidnom gelu u aparatu za automatsko određivanje dužine fragmenta DNA. Svim ispitanicama prethodno su određeni geni lokusa HLA-DRB1 metodm PCR-SSO (Sequence Specific Oligos). Najčešće zastupljeni aleli STR_MICA među istraživanim skupinama bolesnika bili su: DMT1-STR_MICA-A6 (33,7%), APS3v-STR_MICA-A5.1 (31,8%) i AITD-STR_MICA-A5.1 i STR_MICA-A6 (26,2%). Značajnu razliku u učestalosti alela STR_MICA između bolesnika i kontrole pokazao je samo alel STR_MICA-A5 među AITD bolesnicima (12,0 vs. 6,4%; p=0,05). Geni HLA-DRB104 i HLA-DRB103 su statistički značajno češće zastupljeni među bolesnicima s DMT1 i APS3V-om, a samo gen HLA-DRB1*04 među bolesnicima AITD-om. Raspodjela alela STR_MICA u istraživanim bolestima ovisna je o genima HLA-DRB1.
Abstract (english)	The aim of the present study was to investigate the polymorphism of STR_MICA alleles among patients with type 1 diabetes (DMT1), autoimmune polyglandular syndrome type 3 (APS3v) and autoimmune thyroid diseases (AITD), and to determine whether the association between STR_MICA alleles and these diseases is independent or result of linkage disequilibrium with HLA-DRB1 genes. Two hundred and ninety unrelated patients were included (DMT1-86, APS3v-63, and AITD-141). Eighty-six unrelated healthy matched individuals served as control. All samples were tested by polymerase chain reaction (PCR) and ran on 6% polyacrylamide gel in an automated machine for determination of length of alleles, while DRB1 genes were typed by PCR-SSO (Sequence Specific Oligos) method. The most frequent STR_MICA alleles were as follows: DMT1-STR_MICA-A6 (33.7%), APS3v-STR_MICA-A5.1 (31.8%), and AITD-STR_MICA-A5.1 and STR_MICA-A6 (26.2%, each). Only STR_MICA-A5 allele showed significant difference (p=0.05) between AITD patients and controls (12.0 vs. 6.4%). HLA-DRB104 and HLA-DRB103 genes were significantly more present among DMT1 patients and APS3V patients, while among AITD patients only HLA-DRB1*04 gene reached significant p value. The distribution of STR_MICA alleles in investigated diseases is result of linkage disequilibrium with HLA-DRB1 genes.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:420966
Study programme	Title: Experimental Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Science
Created on	2017-04-20 10:47:35

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